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Team:WHU-China/Notebook
From 2011.igem.org
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• Dealing with biobricks</br> | • Dealing with biobricks</br> | ||
• Preparation of competent cells (Using Calcium Chloride)</br> | • Preparation of competent cells (Using Calcium Chloride)</br> | ||
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| - | + | <a href="https://2011.igem.org/Team:WHU-China/Notebook/Progress"><img style="position:absolute;top:380px;right:100px;width:90px;height:30px" src="/wiki/images/1/14/Whu-More.png "></a> | |
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Revision as of 11:22, 28 September 2011
• Dealing with biobricks
• Preparation of competent cells (Using Calcium Chloride)
• Plasmid Extraction（Using Plasmid Mini Kit）
• Transformation (heat-shock method)
• Cleavage with two restriction enzymes
• Get Extraction
• Identification of DNA by Electrophoresis
• Ligation
• PCR of the colony
• Identification of DNA by Sequencing
• Others............
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