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Team:UC Davis/Data LambdacI
From 2011.igem.org
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The above widget displays the relative GFP fluorescence data that we have collected for the eight lambda cI mutant promoters and the wild type R0051 promoter. The plots are normalized to the wild type such that the wild type's fluorescence at 0% arabinose and 0 mM IPTG is 1. The data for these plots were gathered using the constructs outlined on the <a href="https://2011.igem.org/Team:UC_Davis/cilambda">lambda cI project page</a>, which were designed so that increasing the arabinose concentration would proportionally increase the repressor concentration. This widget allows you to toggle the display of whichever mutants you would like to view and easily observe the changes that these mutations cause. | The above widget displays the relative GFP fluorescence data that we have collected for the eight lambda cI mutant promoters and the wild type R0051 promoter. The plots are normalized to the wild type such that the wild type's fluorescence at 0% arabinose and 0 mM IPTG is 1. The data for these plots were gathered using the constructs outlined on the <a href="https://2011.igem.org/Team:UC_Davis/cilambda">lambda cI project page</a>, which were designed so that increasing the arabinose concentration would proportionally increase the repressor concentration. This widget allows you to toggle the display of whichever mutants you would like to view and easily observe the changes that these mutations cause. | ||
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Revision as of 09:55, 28 September 2011
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λ cI Promoter Characterization Data
Wildtype
SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.99566, 0.95671, 0.880328, 0.734557, 0.662629, 0.607491, 0.530352 0.012905, 0.015917, 0.020404, 0.026943, 0.013849, 0.0068952, 0.0060456 4Mutant 1
SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.6492, 0.20526, 0.17329, 0.16899, 0.16682, 0.16631, 0.16913 0.017682, 0.0032754, 0.0010138, 0.0011266, 0.00097033, 0.0012072, 0.0011179Mutant 2
SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.53303, 0.16064, 0.14171, 0.12149, 0.12547, 0.11146, 0.1061 0.02651, 0.0029121, 0.0027198, 0.0033112, 0.0034421, 0.0059837, 0.0027095Mutant 4
SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.23, 0.038825, 0.035663, 0.036119, 0.035423, 0.034289, 0.032858 0.02048, 0.00047265, 0.00028731, 0.00037973, 0.00031652, 0.00026622, 0.00070058Mutant 5
SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.92311, 0.23346, 0.1833, 0.17092, 0.16534, 0.16473, 0.15538 0.0061434, 0.0029621, 0.0016015, 0.0037633, 0.0029015, 0.002096, 0.0024785Mutant 6
SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.97182, 0.98592, 1.01000, 1.02300, 1.03610, 1.04490, 1.01750 0.018621, 0.01966, 0.018634, 0.018826, 0.018993, 0.020762, 0.019842Mutant 7
SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.48325, 0.13919, 0.12353, 0.10617, 0.10943, 0.099213, 0.08565 0.012073, 0.0025601, 0.0030506, 0.0027667, 0.002179, 0.0022998, 0.0015159Mutant 8
SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.41657, 0.12325, 0.10744, 0.10365, 0.10006, 0.096006, 0.09219 0.0080566, 0.0028055, 0.0027614, 0.0038199, 0.0038188, 0.0024541, 0.0022523
The above widget displays the relative GFP fluorescence data that we have collected for the eight lambda cI mutant promoters and the wild type R0051 promoter. The plots are normalized to the wild type such that the wild type's fluorescence at 0% arabinose and 0 mM IPTG is 1. The data for these plots were gathered using the constructs outlined on the lambda cI project page, which were designed so that increasing the arabinose concentration would proportionally increase the repressor concentration. This widget allows you to toggle the display of whichever mutants you would like to view and easily observe the changes that these mutations cause.
