Team:UC Davis/cilambda

From 2011.igem.org

(Difference between revisions)
 
(6 intermediate revisions not shown)
Line 19: Line 19:
<h1>λ cI</h1>
<h1>λ cI</h1>
<div class="floatbox3">
<div class="floatbox3">
-
This promoter/repressor pair originates from Lambda phage. Employing either a lytic or lysogenic life cycle, this bacteriophage infects it's E. coli host with double stranded DNA. cI binds at OR1, OR2 and OR3 sites with preference given to the OR1 site.
+
<a href="http://vimeo.com/29301077"><iframe src="http://player.vimeo.com/video/29301077?title=0&amp;byline=0&amp;portrait=0" align="left" style="margin-right:15px" width="400" height="300" frameborder="0" webkitAllowFullScreen allowFullScreen></iframe></a>
-
</div>
+
This promoter/repressor pair originates from Lambda phage. Employing either a lytic or lysogenic life cycle, this bacteriophage infects its E. coli host with double stranded DNA. cI binds at OR1, OR2 and OR3 sites with preference given to the OR1 site.
-
<div class="floatbox3">
+
-
<center>
+
-
<iframe src="http://player.vimeo.com/video/29301077?title=0&amp;byline=0&amp;portrait=0" width="400" height="300" frameborder="0" webkitAllowFullScreen allowFullScreen></iframe><p><a href="http://vimeo.com/29301077">cI Lambda</a></p>
+
-
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K611007"><img src="https://static.igem.org/mediawiki/2011/7/76/UCD_ci_binding.gif">
+
-
<br><br>
+
-
cI Lambda binding region
+
</div>
</div>
 +
</div>
</div>
</center>
</center>
Line 33: Line 28:
<div class="floatbox">
<div class="floatbox">
<div class="floatbox2">
<div class="floatbox2">
-
<h1>Construct</h1>
+
<h2>Construct</h2>
<center>
<center>
<a href="http://partsregistry.org/Part:BBa_K611017"><img src="https://static.igem.org/mediawiki/2011/a/a5/UCD_R51mut_construct.png">
<a href="http://partsregistry.org/Part:BBa_K611017"><img src="https://static.igem.org/mediawiki/2011/a/a5/UCD_R51mut_construct.png">
Line 42: Line 37:
<div class="floatbox">
<div class="floatbox">
<div class="floatbox2">
<div class="floatbox2">
-
<a href="http://farm7.static.flickr.com/6014/6190729685_171b292c18_b.jpg"><img src="http://farm7.static.flickr.com/6014/6190729685_171b292c18_b.jpg" width="400" height="212">
+
<h2>Mutant Screening</h2>
 +
<center>
 +
<a href="http://farm7.static.flickr.com/6014/6190729685_171b292c18_b.jpg"><img src="http://farm7.static.flickr.com/6014/6190729685_171b292c18_b.jpg" width="400" height="212"></a>
 +
</center>
 +
We selected mutants by first visually picking colonies from transformation plates.  From there we used our plate reader to read the GFP fluorescence of each mutant on a 96 well screening plate.  In the graph above, the green bars represent mutant expression that is 1.5 standard deviations from the average wildtype fluorescence.  Many of our mutants show very low to no expression which indicates that the error-prone pcr which produced them was notably mutagenic. We found 9 suitable candidates to further characterize using the process outlined in the <a href="https://2011.igem.org/Team:UC_Davis/Data"> Data page.</a>
 +
 
</div>
</div>
</div>
</div>

Latest revision as of 09:00, 28 September 2011

Our Sponsors

Start a Family

Got a favorite BioBrick? Check our our process for expanding basic parts into part families.

Criteria

View our judging criteria for iGEM 2011 here.

λ cI

This promoter/repressor pair originates from Lambda phage. Employing either a lytic or lysogenic life cycle, this bacteriophage infects its E. coli host with double stranded DNA. cI binds at OR1, OR2 and OR3 sites with preference given to the OR1 site.

Construct

Mutant Screening

We selected mutants by first visually picking colonies from transformation plates. From there we used our plate reader to read the GFP fluorescence of each mutant on a 96 well screening plate. In the graph above, the green bars represent mutant expression that is 1.5 standard deviations from the average wildtype fluorescence. Many of our mutants show very low to no expression which indicates that the error-prone pcr which produced them was notably mutagenic. We found 9 suitable candidates to further characterize using the process outlined in the Data page.

Retrieved from "http://2011.igem.org/Team:UC_Davis/cilambda"