Team:UNAM-Genomics Mexico/Notebook/pBBR1MCS-5

From 2011.igem.org

(Difference between revisions)
Line 12: Line 12:
At beginning of the week was decided the best way to insert a prefix-RFP-suffix fragment to thepBBR1MCS-5 plasmid, which is a plasmid with broad host-range and is capable to conjugate to R. etli through E. coli  S17, being the most efficient way to introduce genes into R. eltli.  The pBBR1MCS-5 has a multiple cloning site (MCS) with the following restriction sites:
At beginning of the week was decided the best way to insert a prefix-RFP-suffix fragment to thepBBR1MCS-5 plasmid, which is a plasmid with broad host-range and is capable to conjugate to R. etli through E. coli  S17, being the most efficient way to introduce genes into R. eltli.  The pBBR1MCS-5 has a multiple cloning site (MCS) with the following restriction sites:
 +
 +
KpnI
KpnI
 +
 +
ApaI
ApaI
 +
XhoI
XhoI
Sal I
Sal I

Revision as of 07:50, 28 September 2011

UNAM-Genomics_Mexico


Contents


Lab Logbook -pBBR1MCS.

Week: june 13 to 18

At beginning of the week was decided the best way to insert a prefix-RFP-suffix fragment to thepBBR1MCS-5 plasmid, which is a plasmid with broad host-range and is capable to conjugate to R. etli through E. coli S17, being the most efficient way to introduce genes into R. eltli. The pBBR1MCS-5 has a multiple cloning site (MCS) with the following restriction sites:


KpnI


ApaI

XhoI Sal I Bsp106 I ClaI HindIII EcoR1 PstI SmaI Bam H1 SpeI XbaI BstXI SacI