"
Team:UNAM-Genomics Mexico/Notebook/pBBR1MCS-5
From 2011.igem.org
(Difference between revisions)
| Line 5: | Line 5: | ||
| - | = | + | =Lab Logbook -pBBR1MCS.= |
Revision as of 07:49, 28 September 2011
Contents |
Lab Logbook -pBBR1MCS.
Week: june 13 to 18
At beginning of the week was decided the best way to insert a prefix-RFP-suffix fragment to thepBBR1MCS-5 plasmid, which is a plasmid with broad host-range and is capable to conjugate to R. etli through E. coli S17, being the most efficient way to introduce genes into R. eltli. The pBBR1MCS-5 has a multiple cloning site (MCS) with the following restriction sites: KpnI ApaI XhoI Sal I Bsp106 I ClaI HindIII EcoR1 PstI SmaI Bam H1 SpeI XbaI BstXI SacI
