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Team:Brown-Stanford/Lab/Protocols/PhusionPCR
From 2011.igem.org
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=='''Phusion® PCR'''== | =='''Phusion® PCR'''== | ||
| - | (Taken from the [http://www.finnzymes.com/pdf/f530sl_phusion_datasheet_2_2_low.pdf Phusion® High-Fidelity DNA Polymerase Protocol]) | + | (Taken from the [http://www.finnzymes.com/pdf/f530sl_phusion_datasheet_2_2_low.pdf Phusion® High-Fidelity DNA Polymerase Protocol], but edited for amplification of [http://partsregistry.org/Part:BBa_K656013 Part BBa_K656013]) |
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| + | '''Reagents for 20 µL reactions''' | ||
| + | * 4µL 5x Phusion® HF Buffer | ||
| + | * .4 µL 10 mM dNTP | ||
| + | * x µL primer A (.5µM concentration recommended) | ||
| + | * x µL primer B (.5µM concentration recommended) | ||
| + | * x µL template DNA | ||
| + | * .6µL DMSO (optional; recommended for GC-rich amplicons) | ||
| + | * .2µL Phusion® DNA Polymerase | ||
Revision as of 07:18, 28 September 2011
Phusion® PCR
(Taken from the [http://www.finnzymes.com/pdf/f530sl_phusion_datasheet_2_2_low.pdf Phusion® High-Fidelity DNA Polymerase Protocol], but edited for amplification of [http://partsregistry.org/Part:BBa_K656013 Part BBa_K656013])
Reagents for 20 µL reactions
- 4µL 5x Phusion® HF Buffer
- .4 µL 10 mM dNTP
- x µL primer A (.5µM concentration recommended)
- x µL primer B (.5µM concentration recommended)
- x µL template DNA
- .6µL DMSO (optional; recommended for GC-rich amplicons)
- .2µL Phusion® DNA Polymerase
