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Reporter: Week 5 June 12-17
From 2011.igem.org
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===Insert tev Protease into K3 Vector, Day 4=== | ===Insert tev Protease into K3 Vector, Day 4=== | ||
''Sequencing Results:'' The tev protease sequence came back 99% correct. The assembly features a single point mutation at base pair 515: TCT (serine) -> CCT (proline). | ''Sequencing Results:'' The tev protease sequence came back 99% correct. The assembly features a single point mutation at base pair 515: TCT (serine) -> CCT (proline). | ||
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| + | ===Insert Imperial Linker into K3 Vector, Day 4=== | ||
| + | Since we failed to make freezer stock of this correctly assembled part, we transformed the Imp linker + K3 miniprep made for sequencing on Friday, 6/10 into Escherichia coli cells and plated onto kanamycin resistant plates. | ||
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| + | ===Mutagenesis of Xyle, Take 4 Day 1=== | ||
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[[Team:Penn_State/Notebook| Back to Notebook]] | [[Team:Penn_State/Notebook| Back to Notebook]] | ||
Revision as of 14:27, 24 June 2011
Contents |
Sunday
Mutagenesis of Xyle, Take 3 Day 4
The PCR product of the mutated XylE gene from the 4°C fridge was purifed, transformed into Escherichia coli cells and plated onto ampicillin resistant plates.
Monday
Insert tev Protease into K3 Vector, Day 4
Sequencing Results: The tev protease sequence came back 99% correct. The assembly features a single point mutation at base pair 515: TCT (serine) -> CCT (proline).
Insert Imperial Linker into K3 Vector, Day 4
Since we failed to make freezer stock of this correctly assembled part, we transformed the Imp linker + K3 miniprep made for sequencing on Friday, 6/10 into Escherichia coli cells and plated onto kanamycin resistant plates.
