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Reporter: Week 5 June 12-17
From 2011.igem.org
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==Monday== | ==Monday== | ||
===Insert tev Protease into K3 Vector, Day 4=== | ===Insert tev Protease into K3 Vector, Day 4=== | ||
| - | ''Sequencing Results:'' The tev protease sequence came back 99% correct. The assembly features a single point mutation at | + | ''Sequencing Results:'' The tev protease sequence came back 99% correct. The assembly features a single point mutation at base pair 515: TCT (serine) -> CCT (proline). |
[[Team:Penn_State/Notebook| Back to Notebook]] | [[Team:Penn_State/Notebook| Back to Notebook]] | ||
Revision as of 14:23, 24 June 2011
Contents |
Sunday
Mutagenesis of Xyle, Take 3 Day 4
The PCR product of the mutated XylE gene from the 4°C fridge was purifed, transformed into Escherichia coli cells and plated onto ampicillin resistant plates.
Monday
Insert tev Protease into K3 Vector, Day 4
Sequencing Results: The tev protease sequence came back 99% correct. The assembly features a single point mutation at base pair 515: TCT (serine) -> CCT (proline).
