Sensor: July 30 - September 27
From 2011.igem.org
(Created page with "<html> 8/1 </br> - Digest:</br> o OR (E +S)</br> o 34 Cro (E +X)</br> - Grow up:</br> o 34 TEV + B0015</br> o K3</br> - Insert PCR:</br> o OR + 34Cro</br> - PCR:</br> o C0051 + ...") |
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</br></br> | </br></br> | ||
8/2</br> | 8/2</br> | ||
- | - Digest: | + | - Digest:</br> |
- | o Or+34 Cro -> E + S | + | o Or+34 Cro -> E + S</br> |
- | o 34 EV + B0015 (vector) -> E + X | + | o 34 EV + B0015 (vector) -> E + X</br> |
- | o K3 (vector) -> X + P | + | o K3 (vector) -> X + P</br> |
- | o B0030 + C0051 -> X + P | + | o B0030 + C0051 -> X + P</br> |
- | o B0031 + C0051 -> X + P | + | o B0031 + C0051 -> X + P</br> |
- | o B0034 + C0051 -> X + P | + | o B0034 + C0051 -> X + P</br> |
- | o Or + K648000 -> E + X (vector) | + | o Or + K648000 -> E + X (vector)</br> |
- | o B0030 + C0051 -> E + S | + | o B0030 + C0051 -> E + S</br> |
- | o B0031 + C0051 -> E + S | + | o B0031 + C0051 -> E + S</br> |
- | o B0034 + C0051 -> E + S | + | o B0034 + C0051 -> E + S</br> |
- | - Ligated and Plated: | + | - Ligated and Plated:</br> |
- | o B0030+C0051+K3 | + | o B0030+C0051+K3</br> |
- | o B0031+C0051+K3 | + | o B0031+C0051+K3</br> |
- | o B0034+C0051+K3 | + | o B0034+C0051+K3</br> |
- | o Or+34 Cro+34 TEV+B0015 (A) | + | o Or+34 Cro+34 TEV+B0015 (A)</br> |
- | o Or+34 Cro+34 TEV+B0015 (B) | + | o Or+34 Cro+34 TEV+B0015 (B)</br> |
- | o B0030+C0051+Or+K648000 | + | o B0030+C0051+Or+K648000</br> |
- | o B0031+C0051+Or+K648000 | + | o B0031+C0051+Or+K648000</br> |
- | o B0034+C0051+Or+K648000 | + | o B0034+C0051+Or+K648000</br> |
- | 8/3 | + | </br></br> |
- | - Colony PCR: | + | 8/3</br> |
- | o B0030+C0051+K3 | + | - Colony PCR:</br> |
- | o B0031+C0051+K3 | + | o B0030+C0051+K3</br> |
- | o B0034+C0051+K3 | + | o B0031+C0051+K3</br> |
- | o Or+34 Cro+34 TEV+B0015 (A) | + | o B0034+C0051+K3</br> |
- | o Or+34 Cro+34 TEV+B0015 (B) | + | o Or+34 Cro+34 TEV+B0015 (A)</br> |
- | o B0030+C0051+Or+K648000 | + | o Or+34 Cro+34 TEV+B0015 (B)</br> |
- | o B0031+C0051+Or+K648000 | + | o B0030+C0051+Or+K648000</br> |
- | o B0034+C0051+Or+K648000 | + | o B0031+C0051+Or+K648000</br> |
- | o B0030+C0051+K3 | + | o B0034+C0051+Or+K648000</br> |
- | 8/4 | + | o B0030+C0051+K3</br> |
- | - Minipepped an sent to Sequencing: | + | </br></br> |
- | o B0034+C0051+Or+K648000 (R, F) | + | 8/4</br> |
- | o B0030+C0051+K3 (B) | + | - Minipepped an sent to Sequencing:</br> |
- | o B0030+C0051+K3 | + | o B0034+C0051+Or+K648000 (R, F)</br> |
- | o B0030+C0051+Or+K648000 (F, R) | + | o B0030+C0051+K3 (B)</br> |
- | o B0030+C0051+Or+K648000 | + | o B0030+C0051+K3</br> |
- | o B0034+C0051+K3 | + | o B0030+C0051+Or+K648000 (F, R)</br> |
- | o Or+34 Cro+34 TEV+B0015 (B) | + | o B0030+C0051+Or+K648000</br> |
- | o B0021+C0051+K3 | + | o B0034+C0051+K3</br> |
- | o Or+34 Cro+34 TEV+B0015 (A) | + | o Or+34 Cro+34 TEV+B0015 (B)</br> |
- | o Test A | + | o B0021+C0051+K3</br> |
- | o Test B | + | o Or+34 Cro+34 TEV+B0015 (A)</br> |
- | - Digest: | + | o Test A</br> |
- | o B0030+C0051 -> E + S | + | o Test B</br> |
- | o B0031+C0051 -> E + S | + | - Digest:</br> |
- | o B0034+C0051 -> E + S | + | o B0030+C0051 -> E + S</br> |
- | o Or+34 Cro+34 TEV+B0015 -> (vector) E + X | + | o B0031+C0051 -> E + S</br> |
- | - Ligation: | + | o B0034+C0051 -> E + S</br> |
- | o B0030+C0051+Or+34 Cro+34 TEV+B0015 (A + B) | + | o Or+34 Cro+34 TEV+B0015 -> (vector) E + X</br> |
- | o B0031+C0051+Or+24 Cro+34 TEV+B0015 (A + B) | + | - Ligation:</br> |
- | o B0034+C0051+Or+34 Cro+34 TEV+B0015 (A + B) | + | o B0030+C0051+Or+34 Cro+34 TEV+B0015 (A + B)</br> |
- | - Tranform | + | o B0031+C0051+Or+24 Cro+34 TEV+B0015 (A + B)</br> |
- | - Stock = (Done) | + | o B0034+C0051+Or+34 Cro+34 TEV+B0015 (A + B)</br> |
- | o B0030+C0051+K3 | + | - Tranform</br> |
- | o B0031+C0051+K3 | + | - Stock = (Done)</br> |
- | o B0034+C0051+K3 | + | o B0030+C0051+K3</br> |
- | 8/5 | + | o B0031+C0051+K3</br> |
- | - Digest: | + | o B0034+C0051+K3</br> |
- | o Or+34 Cro -> E + S | + | </br></br> |
- | o 34 TEV + B0015 -> E + X (vector) | + | 8/5</br> |
- | o K3 -> X + P (vector) | + | - Digest:</br> |
- | o B0030+C0051 -> X + P | + | o Or+34 Cro -> E + S</br> |
- | o B0031+C0051 -> X + P | + | o 34 TEV + B0015 -> E + X (vector)</br> |
- | o B0034+C0051 -> X + P | + | o K3 -> X + P (vector)</br> |
- | o Or + K648000 -> E + X | + | o B0030+C0051 -> X + P</br> |
- | o B0030+C0051 -> E + S | + | o B0031+C0051 -> X + P</br> |
- | o B0031+C0051 -> E + S | + | o B0034+C0051 -> X + P</br> |
- | o B0034+C0051 -> E + S | + | o Or + K648000 -> E + X</br> |
- | - Ligation: | + | o B0030+C0051 -> E + S</br> |
- | o B0030+C0051+K3 | + | o B0031+C0051 -> E + S</br> |
- | o B0031+C0051+K3 | + | o B0034+C0051 -> E + S</br> |
- | o B0034+C0051+K3 | + | - Ligation:</br> |
- | o Or+34 Cro+34 TEV+B0015 | + | o B0030+C0051+K3</br> |
- | o B0030+C0051+Or+K648000 | + | o B0031+C0051+K3</br> |
- | o B0031+C0051+Or+K648000 | + | o B0034+C0051+K3</br> |
- | o Or+34 Cro+34 TEV+B0015 | + | o Or+34 Cro+34 TEV+B0015</br> |
- | o B0030+C0051+Or+K648000 | + | o B0030+C0051+Or+K648000</br> |
- | o B0031+C0051+Or+K648000 | + | o B0031+C0051+Or+K648000</br> |
- | o B0034+C0051+Or+K648000 | + | o Or+34 Cro+34 TEV+B0015</br> |
- | - Transformed and Plated – all the above | + | o B0030+C0051+Or+K648000</br> |
- | + | o B0031+C0051+Or+K648000</br> | |
- | 8/7 | + | o B0034+C0051+Or+K648000</br> |
- | - Grew colonies from all that was plated on 8/5 | + | - Transformed and Plated – all the above</br> |
- | 8/26 | + | </br></br> |
- | - Digest: (R=Reverse) | + | 8/7</br> |
- | o Or+34 Cro+34 TEV+B0015 -> E + X -> Phosphatase | + | - Grew colonies from all that was plated on 8/5</br> |
- | o B0030+C0051 -> E + S | + | 8/26</br> |
- | o B0031+C0051 -> E + S | + | - Digest: (R=Reverse)</br> |
- | o B0034+C0051 -> E + S | + | o Or+34 Cro+34 TEV+B0015 -> E + X -> Phosphatase</br> |
- | - Ligated, Transformed, and Plated: | + | o B0030+C0051 -> E + S</br> |
- | o B0030+C0051+Or+34 Cro+34 TEV+B0015 | + | o B0031+C0051 -> E + S</br> |
- | o B0031+C0051+Or+34 Cro+34 TEV+B0015 | + | o B0034+C0051 -> E + S</br> |
- | o B0034+C0051+Or+34 Cro+34 TEV+B0015 | + | - Ligated, Transformed, and Plated:</br> |
- | 8/28 | + | o B0030+C0051+Or+34 Cro+34 TEV+B0015</br> |
- | - Grew up: | + | o B0031+C0051+Or+34 Cro+34 TEV+B0015</br> |
- | o B0030+C0051+Or+34 Cro+34 TEV+B0015 | + | o B0034+C0051+Or+34 Cro+34 TEV+B0015</br> |
- | o B0034+C0051+Or+34 Cro+34 TEV+B0015 | + | </br></br> |
- | 8/29 | + | 8/28</br> |
- | - Miniprep and Sequence: | + | - Grew up:</br> |
- | o B0030+C0051+Or+34 Cro+34 TEV+B0015 | + | o B0030+C0051+Or+34 Cro+34 TEV+B0015</br> |
- | o B0034+C0051+Or+34 Cro+34 TEV+B0015 | + | o B0034+C0051+Or+34 Cro+34 TEV+B0015</br> |
- | - 1:1 Molar Solution: | + | </br></br> |
- | o 660 per base | + | 8/29</br> |
- | 9/9 | + | - Miniprep and Sequence:</br> |
- | - Digest: | + | o B0030+C0051+Or+34 Cro+34 TEV+B0015</br> |
- | o B0030+C0051 -> X + S | + | o B0034+C0051+Or+34 Cro+34 TEV+B0015</br> |
- | o B0031+C0051 -> X + S | + | - 1:1 Molar Solution:</br> |
- | o B0034+C0051 -> X + S | + | o 660 per base</br> |
- | - Ran a gel: | + | </br></br> |
- | o Ladder | + | 9/9</br> |
- | o B0030+C0051 | + | - Digest:</br> |
- | o B0031+C0051 | + | o B0030+C0051 -> X + S</br> |
- | o Or+34 Co+34 TEV+B0015 | + | o B0031+C0051 -> X + S</br> |
- | o B0034+C0051 | + | o B0034+C0051 -> X + S</br> |
- | - Extracted the DNA from gel and nanodropped before ligating | + | - Ran a gel:</br> |
- | 9/16 | + | o Ladder</br> |
- | - Miniprepped and PCR’d: | + | o B0030+C0051</br> |
- | o B0030+C0051 (Primers used -> 17679 + 17680) | + | o B0031+C0051</br> |
- | o B0031+C0051 (Primers used -> 17681) | + | o Or+34 Co+34 TEV+B0015</br> |
- | o B0034+C0051 (Primers used -> 17682) | + | o B0034+C0051</br> |
- | - Tecan testing Or | + | - Extracted the DNA from gel and nanodropped before ligating</br> |
- | o Or Mcherry 1.71 -> 6µL | + | </br></br> |
- | D 2 3 4 | + | 9/16</br> |
- | o Or K648000 1.44 -> 7µL | + | - Miniprepped and PCR’d:</br> |
- | E 2 3 4 | + | o B0030+C0051 (Primers used -> 17679 + 17680)</br> |
- | o Or K648000 1.02 -> 10µL | + | o B0031+C0051 (Primers used -> 17681)</br> |
- | F 2 3 4 | + | o B0034+C0051 (Primers used -> 17682)</br> |
- | o B2 .40 -> 25µL | + | - Tecan testing Or</br> |
- | o B4 .8 -> 12 µL | + | o Or Mcherry 1.71 -> 6µL</br> |
- | o B6 .65 -> 15 µL | + | D 2 3 4</br> |
- | o D2 .7 -> 14 µL | + | o Or K648000 1.44 -> 7µL</br> |
- | o D4 .8 -> 12 µL | + | E 2 3 4</br> |
- | o D6 .75 -> 13 µL | + | o Or K648000 1.02 -> 10µL</br> |
- | o F2 .4 -> 11 µL | + | F 2 3 4</br> |
- | o F4 .94 -> 11 µL | + | o B2 .40 -> 25µL</br> |
- | o F6 .9 -> 11 µL | + | o B4 .8 -> 12 µL</br> |
- | + | o B6 .65 -> 15 µL</br> | |
+ | o D2 .7 -> 14 µL</br> | ||
+ | o D4 .8 -> 12 µL</br> | ||
+ | o D6 .75 -> 13 µL</br> | ||
+ | o F2 .4 -> 11 µL</br> | ||
+ | o F4 .94 -> 11 µL</br> | ||
+ | o F6 .9 -> 11 µL</br> | ||
+ | </br></br> | ||
</html> | </html> |
Latest revision as of 06:15, 28 September 2011
8/1 - Digest: o OR (E +S) o 34 Cro (E +X) - Grow up: o 34 TEV + B0015 o K3 - Insert PCR: o OR + 34Cro - PCR: o C0051 + B0030 (R) o C0051 + B0031 (R) o C0051 + B0034 (R) 0 1 4 - Primers used: o 0A o 1A o 4A 8/2 - Digest: o Or+34 Cro -> E + S o 34 EV + B0015 (vector) -> E + X o K3 (vector) -> X + P o B0030 + C0051 -> X + P o B0031 + C0051 -> X + P o B0034 + C0051 -> X + P o Or + K648000 -> E + X (vector) o B0030 + C0051 -> E + S o B0031 + C0051 -> E + S o B0034 + C0051 -> E + S - Ligated and Plated: o B0030+C0051+K3 o B0031+C0051+K3 o B0034+C0051+K3 o Or+34 Cro+34 TEV+B0015 (A) o Or+34 Cro+34 TEV+B0015 (B) o B0030+C0051+Or+K648000 o B0031+C0051+Or+K648000 o B0034+C0051+Or+K648000 8/3 - Colony PCR: o B0030+C0051+K3 o B0031+C0051+K3 o B0034+C0051+K3 o Or+34 Cro+34 TEV+B0015 (A) o Or+34 Cro+34 TEV+B0015 (B) o B0030+C0051+Or+K648000 o B0031+C0051+Or+K648000 o B0034+C0051+Or+K648000 o B0030+C0051+K3 8/4 - Minipepped an sent to Sequencing: o B0034+C0051+Or+K648000 (R, F) o B0030+C0051+K3 (B) o B0030+C0051+K3 o B0030+C0051+Or+K648000 (F, R) o B0030+C0051+Or+K648000 o B0034+C0051+K3 o Or+34 Cro+34 TEV+B0015 (B) o B0021+C0051+K3 o Or+34 Cro+34 TEV+B0015 (A) o Test A o Test B - Digest: o B0030+C0051 -> E + S o B0031+C0051 -> E + S o B0034+C0051 -> E + S o Or+34 Cro+34 TEV+B0015 -> (vector) E + X - Ligation: o B0030+C0051+Or+34 Cro+34 TEV+B0015 (A + B) o B0031+C0051+Or+24 Cro+34 TEV+B0015 (A + B) o B0034+C0051+Or+34 Cro+34 TEV+B0015 (A + B) - Tranform - Stock = (Done) o B0030+C0051+K3 o B0031+C0051+K3 o B0034+C0051+K3 8/5 - Digest: o Or+34 Cro -> E + S o 34 TEV + B0015 -> E + X (vector) o K3 -> X + P (vector) o B0030+C0051 -> X + P o B0031+C0051 -> X + P o B0034+C0051 -> X + P o Or + K648000 -> E + X o B0030+C0051 -> E + S o B0031+C0051 -> E + S o B0034+C0051 -> E + S - Ligation: o B0030+C0051+K3 o B0031+C0051+K3 o B0034+C0051+K3 o Or+34 Cro+34 TEV+B0015 o B0030+C0051+Or+K648000 o B0031+C0051+Or+K648000 o Or+34 Cro+34 TEV+B0015 o B0030+C0051+Or+K648000 o B0031+C0051+Or+K648000 o B0034+C0051+Or+K648000 - Transformed and Plated – all the above 8/7 - Grew colonies from all that was plated on 8/5 8/26 - Digest: (R=Reverse) o Or+34 Cro+34 TEV+B0015 -> E + X -> Phosphatase o B0030+C0051 -> E + S o B0031+C0051 -> E + S o B0034+C0051 -> E + S - Ligated, Transformed, and Plated: o B0030+C0051+Or+34 Cro+34 TEV+B0015 o B0031+C0051+Or+34 Cro+34 TEV+B0015 o B0034+C0051+Or+34 Cro+34 TEV+B0015 8/28 - Grew up: o B0030+C0051+Or+34 Cro+34 TEV+B0015 o B0034+C0051+Or+34 Cro+34 TEV+B0015 8/29 - Miniprep and Sequence: o B0030+C0051+Or+34 Cro+34 TEV+B0015 o B0034+C0051+Or+34 Cro+34 TEV+B0015 - 1:1 Molar Solution: o 660 per base 9/9 - Digest: o B0030+C0051 -> X + S o B0031+C0051 -> X + S o B0034+C0051 -> X + S - Ran a gel: o Ladder o B0030+C0051 o B0031+C0051 o Or+34 Co+34 TEV+B0015 o B0034+C0051 - Extracted the DNA from gel and nanodropped before ligating 9/16 - Miniprepped and PCR’d: o B0030+C0051 (Primers used -> 17679 + 17680) o B0031+C0051 (Primers used -> 17681) o B0034+C0051 (Primers used -> 17682) - Tecan testing Or o Or Mcherry 1.71 -> 6µL D 2 3 4 o Or K648000 1.44 -> 7µL E 2 3 4 o Or K648000 1.02 -> 10µL F 2 3 4 o B2 .40 -> 25µL o B4 .8 -> 12 µL o B6 .65 -> 15 µL o D2 .7 -> 14 µL o D4 .8 -> 12 µL o D6 .75 -> 13 µL o F2 .4 -> 11 µL o F4 .94 -> 11 µL o F6 .9 -> 11 µL