Team:Panama/Protocols/Wetlab

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18. Cell Culture
18. Cell Culture
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[https://2011.igem.org/Team:Panama '''Home''']

Revision as of 05:43, 28 September 2011

1. Transformation

2. PCR

3. Colony PCR

4. Pgem Cloning

5. Preparing chemically competent cells

6. LB media recipie

7. Ampicilyn stock

8. Chloramfenicol Stock

9. Mutagenesis PCR

10. Mutagenesis transformation

11. Miniprep Quiagen Kit

12. Miniprep "Dirty"

13. PCR purification

14. Glicerol Stock

15. Agarose gel electrophorsis

16. Agar plates

17. Primer aliquots

18. Cell Culture

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