Team:Panama/BioBricks

From 2011.igem.org

(Difference between revisions)
(Re-designing and re-assembling of the rhamnosyltransferase BioBrick part:"The Biosurfactator")
(Re-designing and re-assembling of the rhamnosyltransferase BioBrick part:"The Biosurfactator")
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We developed this standarized part to be inserted into E. coli for rhamnolipid production through the mutation of the gene that is responsible to synthesize the key enzyme rhamnosyltranferase (RhlAB) to be compatible with Assembly Standard 10. We have achieved this by performing several silent mutations using the QuikLighting Multi Site-Directed Mutagenesis Kit from Stratagene. This rhamnosyltransferase BioBrick (Rh1AB_BB) is ready to be tested on an expression plasmid device following the Assembly Standard Protocol 10.
We developed this standarized part to be inserted into E. coli for rhamnolipid production through the mutation of the gene that is responsible to synthesize the key enzyme rhamnosyltranferase (RhlAB) to be compatible with Assembly Standard 10. We have achieved this by performing several silent mutations using the QuikLighting Multi Site-Directed Mutagenesis Kit from Stratagene. This rhamnosyltransferase BioBrick (Rh1AB_BB) is ready to be tested on an expression plasmid device following the Assembly Standard Protocol 10.
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[Our 2011 BioBrick Here: http://partsregistry.org/Part:BBa_K653000:Design]
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[[See our 2011 BioBrick here!]]

Revision as of 03:44, 28 September 2011