Looking to clone RBS into plasmid pSB1A10 which contain RFP. Transformed pSB1A10 from initial distribution. <br/>
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8/9/2011<br/>
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Successful transformation with multiple red colonies. Inoculated pSB1A10. <br/>
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8/10/2011<br/>
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Miniprepped pSB1A10. Digested and ligated RBS's B0030 and B0034 into pSB1A10. Looking to transform tomorrow.<br/>
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8/12/2011<br/>
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Transformed new screening plasmid, pSB1A10, with the RBSs ligated.
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Transformed two T. cruzi radioresistance genes. Hopefully, the same radioresistance that the plant enjoys can be conferred on E. coli through biobricks. <br/>
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Ran PCR and gel to verify E. coli biobrick transformants. Verification primers amplify gene insertion plus ~100 bp in either direction. Samples whose lengths match expected sizes are sent for Sager sequencing.<br/>
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