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Team:Tec-Monterrey/projectnotebook july/day12
From 2011.igem.org
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| - | + | <p class="justtext"> | |
| - | < | + | 12/July/2011 |
| + | </br> | ||
| + | • Minipreps -> 14L col.3, 8G col.3 and 13L8G col 19 | ||
| + | </br> | ||
| + | • Digestion with EcoRI , SpeI and XbaI ; 2m with PstI and SpeI | ||
| + | </br> | ||
| + | • Electrophoresis | ||
| + | </br> | ||
| + | • Chema gave us certain strains for the expression of the protein, which are: | ||
| + | Roset Gami, XL1 Blue, C43, Pet 12, BL21, C41, Pet 28 | ||
| + | </br> | ||
| + | • Purification -> 13L col.3 and 8G col.3 | ||
| + | </br> | ||
| + | • Ligation -> 13L+8G | ||
| + | </br> | ||
| + | • Touchdown PCR for SacC, Program: 61-43ºC, Δ1ºC every 2 cycles. | ||
| + | </br> | ||
| + | • PCR product purification, quantification and overnight ligation in pGEM. | ||
| + | </p> | ||
Revision as of 03:05, 28 September 2011
12/July/2011 • Minipreps -> 14L col.3, 8G col.3 and 13L8G col 19 • Digestion with EcoRI , SpeI and XbaI ; 2m with PstI and SpeI • Electrophoresis • Chema gave us certain strains for the expression of the protein, which are: Roset Gami, XL1 Blue, C43, Pet 12, BL21, C41, Pet 28 • Purification -> 13L col.3 and 8G col.3 • Ligation -> 13L+8G • Touchdown PCR for SacC, Program: 61-43ºC, Δ1ºC every 2 cycles. • PCR product purification, quantification and overnight ligation in pGEM.
