Team:NYC Wetware/Notebook/Week10

From 2011.igem.org

(Difference between revisions)
(Created page with "{{:Team:NYC_Wetware/Templates/nav}} {{:Team:NYC_Wetware/Templates/css}} <html> <html lang="en"> <head> <meta charset="utf-8"> <title>NYC-iGEM wetware</title> <meta name "descrip...")
 
(3 intermediate revisions not shown)
Line 19: Line 19:
<br/>
<br/>
-
7/26/2011 - 8/1/2011<br/>
 
-
Iterative transformation, inoculation, miniprep. Prepared 20+ E. coli genes for assembly into our radioresistance biobricks. <br/>
 
<br/>
<br/>
-
Also, looked into using the screening plasmid, pSB1A10, for future transformations and how to correctly use the pBAD promoter. <br/>
+
Looking to clone RBS into plasmid pSB1A10 which contain RFP. Transformed pSB1A10 from initial distribution.
 +
<br/><br/>
 +
Successful transformation with multiple red colonies. Inoculated pSB1A10. <br/>
<br/>
<br/>
 +
Miniprepped pSB1A10. Digested and ligated RBS's B0030 and B0034 into pSB1A10. Looking to transform tomorrow.<br/>
 +
<br/>
 +
Transformed new screening plasmid, pSB1A10, with the RBSs ligated.
 +
Transformed two T. cruzi radioresistance genes. Hopefully, the same radioresistance that the plant enjoys can be conferred on E. coli through biobricks. <br/>
 +
<br/>
 +
Ran PCR and gel to verify E. coli biobrick transformants. Verification primers amplify gene insertion plus ~100 bp in either direction. Samples whose lengths match expected sizes are sent for Sager sequencing.<br/>

Latest revision as of 02:55, 28 September 2011