Team:Caltech/Week 2

From 2011.igem.org

(Difference between revisions)
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== June 22 ==
== June 22 ==
-
<p>Miniprep and sequencing of selected BioBricks ([[partsregistry.org/Part:BBa_K123000|K123000]], [[partsregistry.org/Part:BBa_K123001|K123001]], [[partsregistry.org/Part:BBa_K123002|K123002]], [[partsregistry.org/Part:BBa_K123003|K123003]]) <br/>
+
<p>Miniprep and sequencing of selected BioBricks ([http://partsregistry.org/Part:BBa_K123000 K123000], [http://partsregistry.org/Part:BBa_K123001 K123001], [http://partsregistry.org/Part:BBa_K123002 K123002], [http://partsregistry.org/Part:BBa_K123003 K123003]) <br/>
Preparation of competent cells</p>  
Preparation of competent cells</p>  
==June 23==
==June 23==
-
<p>Transform T7 polymerase ([[partsregistry.org/Part:BBa_K145001|K145001]]), mCherry ([[partsregistry.org/Part:BBa_J06702|J06702]]), Lac Promoter ([[partsregistry.org/Part:BBa_R0010|R0010]]), and double terminator ([[partsregistry.org/Part:BBa_B0014|B0014]] and [[partsregistry.org/Part:BBa_B0015|B0015]]) biobricks for test sequences for BisA and BisB degradation genes ([[partsregistry.org/Part:BBa_K123000|K123000]] and [[partsregistry.org/Part:BBa_K123001|K123001]])<br/>
+
<p>Transform T7 polymerase ([http://partsregistry.org/Part:BBa_K145001 K145001]), mCherry ([http://partsregistry.org/Part:BBa_J06702 J06702]]), Lac Promoter ([http://partsregistry.org/Part:BBa_R0010 R0010]]), and double terminator ([http://partsregistry.org/Part:BBa_B0014 B0014] and [http://partsregistry.org/Part:BBa_B0015 B0015]) biobricks for test sequences for BisA and BisB degradation genes ([http://partsregistry.org/Part:BBa_K123000 K123000] and [http://partsregistry.org/Part:BBa_K123001 K123001])<br/>
-
Retest competent cells made June 22nd with [[partsregistry.org/Part:BBa_K123001|K123001]] and pUC<br/>
+
Retest competent cells made June 22nd with [http://partsregistry.org/Part:BBa_K123001 K123001] and pUC<br/>
Transfer 0.5 mL aliquots of BPA and 17a-ethynylestradiol cultures from June 20 to new tubes of minimal media<br/>
Transfer 0.5 mL aliquots of BPA and 17a-ethynylestradiol cultures from June 20 to new tubes of minimal media<br/>
Design primers for test sequence of BisdA (pNT001)<br/>
Design primers for test sequence of BisdA (pNT001)<br/>
-
Design primers to continue sequencing of human ER ([[partsregistry.org/Part:BBa_K123003|K123003]])<br/>
+
Design primers to continue sequencing of human ER ([http://partsregistry.org/Part:BBa_K123003 K123003])<br/>
-
We determined that sequences for [[partsregistry.org/Part:BBa_K123000|K123000]] and [[partsregistry.org/Part:BBa_K123001|K123001]] have correct protein coding but different codons than in the sequences shown online </p>
+
We determined that sequences for [http://partsregistry.org/Part:BBa_K123000 K123000] and [http://partsregistry.org/Part:BBa_K123001 K123001] have correct protein coding but different codons than in the sequences shown online </p>
==June 24==
==June 24==
-
<p>Transform Tet repressible promoter [[partsregistry.org/Part:BBa_R0040|R0040]]<br/>
+
<p>Transform Tet repressible promoter [http://partsregistry.org/Part:BBa_R0040 R0040]<br/>
Make plain LB and pour plain LB plates<br/>
Make plain LB and pour plain LB plates<br/>
Make SOC media<br/>
Make SOC media<br/>
Transfer 0.5 mL DDT and nonylphenol cultures from June 21 to new tubes of minimal media <br/>
Transfer 0.5 mL DDT and nonylphenol cultures from June 21 to new tubes of minimal media <br/>
Wash dishes from last year <br/>
Wash dishes from last year <br/>
-
Design reverse primer for continued sequencing of human ER ([[partsregistry.org/Part:BBa_K123003|K123003]]) <br/>
+
Design reverse primer for continued sequencing of human ER ([http://partsregistry.org/Part:BBa_K123003 K123003]) <br/>
Update Wiki with protocols we have been using</p>  
Update Wiki with protocols we have been using</p>  
Line 43: Line 43:
==June 26==
==June 26==
-
<p> Start overnight cultures of T7 polymerase ([[partsregistry.org/Part:BBa_K145001|K145001]]), mCherry ([[partsregistry.org/Part:BBa_J06702|J06702]]), Lac Promoter ([[partsregistry.org/Part:BBa_R0010|R0010]]), double terminator ([[partsregistry.org/Part:BBa_B0014|B0014]] and [[partsregistry.org/Part:BBa_B0015|B0015]]) and Tet Promoter ([[partsregistry.org/Part:BBa_R0040|R0040]])</p>
+
<p> Start overnight cultures of T7 polymerase ([http://partsregistry.org/Part:BBa_K145001 K145001]), mCherry ([http://partsregistry.org/Part:BBa_J06702 J06702]), Lac Promoter ([http://partsregistry.org/Part:BBa_R0010 R0010]), double terminator ([http://partsregistry.org/Part:BBa_B0014 B0014] and [http://partsregistry.org/Part:BBa_B0015 B0015]) and Tet Promoter ([http://partsregistry.org/Part:BBa_R0040 R0040])</p>
}}
}}

Revision as of 03:04, 24 June 2011


Caltech iGEM 2011



Home

Project

Data

Parts

Team

Notebook

Biosafety

Human Impact

References

Support

June 19

Collection of soil and water samples from LA River

June 20

Lab walkthrough
Setting up lab, stock supplies, preparation of LB-amp plates
Test extraction of DNA from BioBrick wells into cloning plates
Initial enrichment cultures of collected samples on minimal media with BPA or 17a-ethynylestradiol

June 21

MoBio kit extraction of DNA from collected samples
Initial enrichment cultures of samples in minimal media with nonylphenol or DDT
Geneious and primer design tutorial

June 22

Miniprep and sequencing of selected BioBricks ([http://partsregistry.org/Part:BBa_K123000 K123000], [http://partsregistry.org/Part:BBa_K123001 K123001], [http://partsregistry.org/Part:BBa_K123002 K123002], [http://partsregistry.org/Part:BBa_K123003 K123003])
Preparation of competent cells

June 23

Transform T7 polymerase ([http://partsregistry.org/Part:BBa_K145001 K145001]), mCherry ([http://partsregistry.org/Part:BBa_J06702 J06702]]), Lac Promoter ([http://partsregistry.org/Part:BBa_R0010 R0010]]), and double terminator ([http://partsregistry.org/Part:BBa_B0014 B0014] and [http://partsregistry.org/Part:BBa_B0015 B0015]) biobricks for test sequences for BisA and BisB degradation genes ([http://partsregistry.org/Part:BBa_K123000 K123000] and [http://partsregistry.org/Part:BBa_K123001 K123001])
Retest competent cells made June 22nd with [http://partsregistry.org/Part:BBa_K123001 K123001] and pUC
Transfer 0.5 mL aliquots of BPA and 17a-ethynylestradiol cultures from June 20 to new tubes of minimal media
Design primers for test sequence of BisdA (pNT001)
Design primers to continue sequencing of human ER ([http://partsregistry.org/Part:BBa_K123003 K123003])
We determined that sequences for [http://partsregistry.org/Part:BBa_K123000 K123000] and [http://partsregistry.org/Part:BBa_K123001 K123001] have correct protein coding but different codons than in the sequences shown online

June 24

Transform Tet repressible promoter [http://partsregistry.org/Part:BBa_R0040 R0040]
Make plain LB and pour plain LB plates
Make SOC media
Transfer 0.5 mL DDT and nonylphenol cultures from June 21 to new tubes of minimal media
Wash dishes from last year
Design reverse primer for continued sequencing of human ER ([http://partsregistry.org/Part:BBa_K123003 K123003])
Update Wiki with protocols we have been using

June 25

Take out plates from June 24

June 26

Start overnight cultures of T7 polymerase ([http://partsregistry.org/Part:BBa_K145001 K145001]), mCherry ([http://partsregistry.org/Part:BBa_J06702 J06702]), Lac Promoter ([http://partsregistry.org/Part:BBa_R0010 R0010]), double terminator ([http://partsregistry.org/Part:BBa_B0014 B0014] and [http://partsregistry.org/Part:BBa_B0015 B0015]) and Tet Promoter ([http://partsregistry.org/Part:BBa_R0040 R0040])


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