Team:Calgary/Notebook/Protocols/Process7

From 2011.igem.org

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<h4> Reagents </h4>
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<li>HEPES (N-[2-hydroxyethyl] piperazine N'[2-ethane-sulfonic acid])</li>
<li>HEPES (N-[2-hydroxyethyl] piperazine N'[2-ethane-sulfonic acid])</li>
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<li>High-speed centrifuge and rotor</li>
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Revision as of 01:47, 28 September 2011


Plasmid Extraction

This protocol will purify plasmids from bacteria, though identification of plasmids will need to be confirmed by PCR.


Reagents

  • HEPES (N-[2-hydroxyethyl] piperazine N'[2-ethane-sulfonic acid])
  • Sorbitol
  • Percoll
  • PEG 6000
  • Ficoll
  • Bovine serum albumin (BSA)
  • Isoascorbic acid
  • Sodium pyrophosphate
  • Glutathione
  • MgCl2.6H2O
  • MnCl2
  • Sodium-EDTA

    • Equipment

  • High-speed centrifuge and rotor
  • Spectrophotometer for chlorophyll measurements
  • Orbital shaker, light suitable for algal cell growth, and timer to control light settings
  • 27-gauge * 0.5 inch stainless stell syringe needles
  • B-D Multifit 10mL glass Leuer-lock syringe
  • 15mL Corex centrifuge tubes
  • Hemocytometer (cell counter)
  • Fine (artist style) paint brush

    • Protocol

    Retrieved from "http://2011.igem.org/Team:Calgary/Notebook/Protocols/Process7"