"
Team:Yale/Notebook/Week8
From 2011.igem.org
(Difference between revisions)
(Created page with "{{:Team:Yale/Templates/Yale_Header_Else}} <html> <div id="text"> <div id="container"> <div id="left-col"> <ul id="nb"> <li><a id="page" href="https://2011.igem.org/Team:Yal...") |
|||
| (3 intermediate revisions not shown) | |||
| Line 5: | Line 5: | ||
<div id="left-col"> | <div id="left-col"> | ||
<ul id="nb"> | <ul id="nb"> | ||
| - | <li><a | + | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week1">Week 1</a></li> |
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week2">Week 2</a></li> | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week2">Week 2</a></li> | ||
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week3">Week 3</a></li> | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week3">Week 3</a></li> | ||
| Line 12: | Line 12: | ||
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week6">Week 6</a></li> | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week6">Week 6</a></li> | ||
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week7">Week 7</a></li> | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week7">Week 7</a></li> | ||
| - | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week8">Week 8</a></li> | + | <li><a id="page" href="https://2011.igem.org/Team:Yale/Notebook/Week8">Week 8</a></li> |
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week9">Week 9</a></li> | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week9">Week 9</a></li> | ||
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week10">Week 10</a></li> | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week10">Week 10</a></li> | ||
| + | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week11">Week 11</a></li> | ||
| + | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week12">Week 12</a></li> | ||
| + | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week13">Week 13</a></li> | ||
| + | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week14">Week 14</a></li> | ||
| + | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week15">Week 15</a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
<div id="entry"> | <div id="entry"> | ||
| - | Week 8 | + | <h1>Week 8: July 3-10, 2011</h1> |
| + | <ul> | ||
| + | <li>Designed and ordered primers with BioBrick prefix and suffix overhangs to PCR amplify following constructs <ul> | ||
| + | <li>RiAFP-His</li> | ||
| + | <li>eGFP-TEV-RiAFP-His</li> | ||
| + | <li>eGFP-TmAFP-His</li> | ||
| + | <li>eGFP-TEV-TmAFP-His</li> | ||
| + | <li>TmAFP-His</li> | ||
| + | </ul> | ||
| + | <li>Set up PCR amplification with Phusion polymerase of aforementioned constructs, verified successful amplification: (show figure)</li> | ||
| + | <li>Digest PCR fragments using XbaI and PstI, digest BioBrick BBa_I712074 (contains T7 polymerase) with SpeI and PstI</li> | ||
| + | <li>Ligate using T4 ligase, transform cells and plate on Amp plates (backbone of plasmid is AmpR and KanR)</li> | ||
| + | <li>Verification of successful ligation using colony PCR</li> | ||
| + | </ul> | ||
</div> | </div> | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
</div> | </div> | ||
Latest revision as of 01:17, 28 September 2011
