Team:UT Dallas/week8

From 2011.igem.org

(Difference between revisions)

Latest revision as of 00:54, 28 September 2011

Week 8

8/21/11	Incubation: GFP

8/22/11	Glycerol Stock/Miniprep GFP Test Digestion + Gel Electrophoresis: GFP Dissolving/Diluting Primers for ctx promoter and FGFR PCR/Gel purification: ctx

8/23/11	Full Digestion: CheZ, CheZ, ctx promoter Dissolving/Diluting Primers: ----------------------------- Gel Electrophoresis/ Purification of CheZ, CheZ Ligation: ctx with CheZ, CheZ*

8/24/11	PCR Point Mutation: FGFR (get rid of EcoRI restriction site) Transformation (XL10 ultra competent): FGFR (point mutated) Transformation: Ctx-CheZ (Carb), ctx-CheZ* (Chlora)

8/25/11	Incubation: FGFR Digestion, Gel Electrophoresis/Purification: CheZ, CheZ* Ligation Overnight: Ctx --> CheZ, CheZ*

8/26/11	Glycerol Stock/Miniprep: FGFR Transformation CheZ, CheZ* Test Digestion, Gel Electrophoresis Made LbB Chlora & LB Kan

8/27/11	Digestion, Gel Electrophoresis/Purification: RBS-TetR, GFP Ligation (10 min): GFP into RBS-TetR-double term Transformation: GFP-RBS-TetR-term

@@ Line 1: / Line 1: @@
 <h2>Week 8</h2>
 <table>
-<tr><td valign='top'>8/28/11</td><td valign='top'>Transformation (DH5-alpha): GFP-RBS-TetR-term<br>
+<tr><td valign='top'>8/21/11</td><td valign='top'>Incubation: GFP</td></tr>
-Incubation of CheZ, CheZ*</td>
 <tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
-<tr><td valign='top'>8/29/11</td><td valign='top'> Glycerol/Miniprep ctx-CheZ, ctx-CheZ*
+<tr><td valign='top'>8/22/11</td><td valign='top'>Glycerol Stock/Miniprep GFP<br>
-Digestion: ToxR, FGFR
+	Test Digestion + Gel Electrophoresis: GFP<br>
-Incubation ToxR</td>
+	Dissolving/Diluting Primers for ctx promoter and FGFR<br>
+	PCR/Gel purification: ctx</td></tr>
 <tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
-<tr><td valign='top'>8/30/11</td><td valign='top'> Test Digestion: ctx-CheZ, ctx-Chez*, CheZ, CheZ*<br>
+<tr><td valign='top'>8/23/11</td><td valign='top'>Full Digestion: CheZ, CheZ*, ctx promoter<br>
-Gel Electrophoresis: ctx-CheZ, ctx-Chez*<br>
+	Dissolving/Diluting Primers: -----------------------------<br>
-Incubation: ToxR</td>
+	Gel Electrophoresis/ Purification of CheZ, CheZ*<br>
+	Ligation: ctx with CheZ, CheZ*</td></tr>
 <tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
-<tr><td valign='top'>8/31/11</td><td valign='top'> Miniprep: ToxR<br>
+<tr><td valign='top'>8/24/11</td><td valign='top'>PCR Point Mutation: FGFR (get rid of EcoRI restriction site)<br>
-Full Digestion: ToxR, pSB1C3<br>
+	Transformation (XL10 ultra competent): FGFR (point mutated) <br>
-Gel Electrophoresis/Purifcation: ToxR,GFP -> CheZ*, FGFR, RBS-TetR-Term<br>
+	Transformation: Ctx-CheZ (Carb), ctx-CheZ* (Chlora)</td></tr>
-Transformation: Ctx-CheZ*, ToxR-FGFR, GFP-RBS-TetR-Term</td>
 <tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
-<tr><td valign='top'>9/1/11</td><td valign='top'> Plating: k422007 CheY<br>
+<tr><td valign='top'>8/25/11</td><td valign='top'>Incubation: FGFR
-Incubation: GFP-RBS-TetR-Term (Carb)<br>
+	Digestion, Gel Electrophoresis/Purification: CheZ, CheZ*<br>
-Incubation: E.Coli FHK12 - from Germany (Carb)<br>
+	Ligation Overnight: Ctx -->  CheZ, CheZ*</td></tr>
-Incubation: CheZ* (Chlora)</td>
 <tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
-<tr><td valign='top'>9/2/11</td><td valign='top'> Plates Made: CarbR, ChloraR, CarbR/ChloraR<br>
+<tr><td valign='top'>8/26/11</td><td valign='top'>Glycerol Stock/Miniprep: FGFR<br>
-Broth Made: LB CarbR, LB Carb/ChloraR<br>
+	Transformation CheZ, CheZ*<br>
-Miniprep: GFP-RBS-TetR-Term, CheZ*<br>
+	Test Digestion, Gel Electrophoresis<br>
-Glycerol Stock: GFP-RBS-TetR-Term<br>
+	Made LbB Chlora & LB Kan</td></tr>
-Made Competent Cells: FHK12 (Carb)<br>
-Test Digestion/Gel Electrophoresis: GFP-RBS-TetR-Term,<br>
-Full Digestion: CheZ*, FGFR<br>
-Ful Digestion: GFP-RBS-TetR-Term<br>
-Transformation: German Parts=[pHKToxR'MalE, pHKToxR'MalE/ZIP, pHKToxR'REI-T39K, pHKToxR<br>
-Plasmid stock: Diluted German parts (as above)<br>
-Ligation 10 Min: ctx, ToxR into CheZ*, FGFR respectively<br>
-Transformation: CheZ*, ctx-CheZ*, FGFR, ToxR-FGFR<br>
-Ligation Overnight: Vectors= GFP-RBS-TetR-Term, PyeaR, scp  <br>
-		 Inserts= ctx, GFP-RBS, TetR-Term</td>
 <tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
-<tr><td valign='top'>9/3/11</td><td valign='top'> Transformation: ctx-GFP-RBS-TetR-Term, PyeaR-GFP-RBS-TetR-Term, scp-GFP-RBS-TetR-Term<br></td>
+<tr><td valign='top'>8/27/11</td><td valign='top'>Digestion, Gel Electrophoresis/Purification: RBS-TetR, GFP<br>
+	Ligation (10 min): GFP into RBS-TetR-double term<br>
+		Transformation: GFP-RBS-TetR-term</td></tr>
 </table>