Team:UT Dallas/week8

From 2011.igem.org

(Difference between revisions)
(Created page with "<h2>Week 8</h2> <table> <tr><td valign='top'>8/28/11</td><td valign='top'>Transformation (DH5-alpha): GFP-RBS-TetR-term<br> Incubation of CheZ, CheZ*</td> <tr><td valign='top'>&n...")
 
(3 intermediate revisions not shown)
Line 1: Line 1:
<h2>Week 8</h2>
<h2>Week 8</h2>
<table>
<table>
-
<tr><td valign='top'>8/28/11</td><td valign='top'>Transformation (DH5-alpha): GFP-RBS-TetR-term<br>
+
<tr><td valign='top'>8/21/11</td><td valign='top'>Incubation: GFP</td></tr>
-
Incubation of CheZ, CheZ*</td>
+
<tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
<tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
-
<tr><td valign='top'>8/29/11</td><td valign='top'> Glycerol/Miniprep ctx-CheZ, ctx-CheZ*
+
<tr><td valign='top'>8/22/11</td><td valign='top'>Glycerol Stock/Miniprep GFP<br>
-
Digestion: ToxR, FGFR
+
Test Digestion + Gel Electrophoresis: GFP<br>
-
Incubation ToxR</td>
+
Dissolving/Diluting Primers for ctx promoter and FGFR<br>
 +
PCR/Gel purification: ctx</td></tr>
<tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
<tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
-
<tr><td valign='top'>8/30/11</td><td valign='top'> Test Digestion: ctx-CheZ, ctx-Chez*, CheZ, CheZ*<br>
+
<tr><td valign='top'>8/23/11</td><td valign='top'>Full Digestion: CheZ, CheZ*, ctx promoter<br>
-
Gel Electrophoresis: ctx-CheZ, ctx-Chez*<br>
+
Dissolving/Diluting Primers: -----------------------------<br>
-
Incubation: ToxR</td>
+
Gel Electrophoresis/ Purification of CheZ, CheZ*<br>
 +
Ligation: ctx with CheZ, CheZ*</td></tr>
<tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
<tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
-
<tr><td valign='top'>8/31/11</td><td valign='top'> Miniprep: ToxR<br>
+
<tr><td valign='top'>8/24/11</td><td valign='top'>PCR Point Mutation: FGFR (get rid of EcoRI restriction site)<br>
-
Full Digestion: ToxR, pSB1C3<br>
+
Transformation (XL10 ultra competent): FGFR (point mutated) <br>
-
Gel Electrophoresis/Purifcation: ToxR,GFP -> CheZ*, FGFR, RBS-TetR-Term<br>
+
Transformation: Ctx-CheZ (Carb), ctx-CheZ* (Chlora)</td></tr>
-
Transformation: Ctx-CheZ*, ToxR-FGFR, GFP-RBS-TetR-Term</td>
+
<tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
<tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
-
<tr><td valign='top'>9/1/11</td><td valign='top'> Plating: k422007 CheY<br>
+
<tr><td valign='top'>8/25/11</td><td valign='top'>Incubation: FGFR
-
Incubation: GFP-RBS-TetR-Term (Carb)<br>
+
Digestion, Gel Electrophoresis/Purification: CheZ, CheZ*<br>
-
Incubation: E.Coli FHK12 - from Germany (Carb)<br>
+
Ligation Overnight: Ctx --> CheZ, CheZ*</td></tr>
-
Incubation: CheZ* (Chlora)</td>
+
<tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
<tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
-
<tr><td valign='top'>9/2/11</td><td valign='top'> Plates Made: CarbR, ChloraR, CarbR/ChloraR<br>
+
<tr><td valign='top'>8/26/11</td><td valign='top'>Glycerol Stock/Miniprep: FGFR<br>
-
Broth Made: LB CarbR, LB Carb/ChloraR<br>
+
Transformation CheZ, CheZ*<br>
-
Miniprep: GFP-RBS-TetR-Term, CheZ*<br>
+
Test Digestion, Gel Electrophoresis<br>
-
Glycerol Stock: GFP-RBS-TetR-Term<br>
+
Made LbB Chlora & LB Kan</td></tr>
-
Made Competent Cells: FHK12 (Carb)<br>
+
-
Test Digestion/Gel Electrophoresis: GFP-RBS-TetR-Term,<br>
+
-
Full Digestion: CheZ*, FGFR<br>
+
-
Ful Digestion: GFP-RBS-TetR-Term<br>
+
-
Transformation: German Parts=[pHKToxR'MalE, pHKToxR'MalE/ZIP, pHKToxR'REI-T39K, pHKToxR<br>
+
-
Plasmid stock: Diluted German parts (as above)<br>
+
-
Ligation 10 Min: ctx, ToxR into CheZ*, FGFR respectively<br>
+
-
Transformation: CheZ*, ctx-CheZ*, FGFR, ToxR-FGFR<br>
+
-
Ligation Overnight: Vectors= GFP-RBS-TetR-Term, PyeaR, scp  <br>
+
-
Inserts= ctx, GFP-RBS, TetR-Term</td>
+
<tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
<tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
-
<tr><td valign='top'>9/3/11</td><td valign='top'> Transformation: ctx-GFP-RBS-TetR-Term, PyeaR-GFP-RBS-TetR-Term, scp-GFP-RBS-TetR-Term</td>
+
<tr><td valign='top'>8/27/11</td><td valign='top'>Digestion, Gel Electrophoresis/Purification: RBS-TetR, GFP<br>
 +
Ligation (10 min): GFP into RBS-TetR-double term<br>
 +
Transformation: GFP-RBS-TetR-term</td></tr>
</table>
</table>

Latest revision as of 00:54, 28 September 2011

Week 8

8/21/11Incubation: GFP
  
8/22/11Glycerol Stock/Miniprep GFP

Test Digestion + Gel Electrophoresis: GFP
Dissolving/Diluting Primers for ctx promoter and FGFR

PCR/Gel purification: ctx
  
8/23/11Full Digestion: CheZ, CheZ*, ctx promoter

Dissolving/Diluting Primers: -----------------------------
Gel Electrophoresis/ Purification of CheZ, CheZ*

Ligation: ctx with CheZ, CheZ*
  
8/24/11PCR Point Mutation: FGFR (get rid of EcoRI restriction site)

Transformation (XL10 ultra competent): FGFR (point mutated)

Transformation: Ctx-CheZ (Carb), ctx-CheZ* (Chlora)
  
8/25/11Incubation: FGFR

Digestion, Gel Electrophoresis/Purification: CheZ, CheZ*

Ligation Overnight: Ctx --> CheZ, CheZ*
  
8/26/11Glycerol Stock/Miniprep: FGFR

Transformation CheZ, CheZ*
Test Digestion, Gel Electrophoresis

Made LbB Chlora & LB Kan
  
8/27/11Digestion, Gel Electrophoresis/Purification: RBS-TetR, GFP

Ligation (10 min): GFP into RBS-TetR-double term

Transformation: GFP-RBS-TetR-term
Retrieved from "http://2011.igem.org/Team:UT_Dallas/week8"