Team:Calgary/Notebook/Protocols/Process14

From 2011.igem.org

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      <td width="85%"><div class="heading"><a style="text-decoration:none" name="rehydration"></a> Rehydration</div></td>
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Revision as of 21:59, 27 September 2011


Rehydration of Registry DNA

Rehydration

Biobrick parts are shipped from the registry in a dehydrated form. As such they must be rehydrated before they can be used.

  1. Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want
  2. Add 10 μL of ddH20 (deionized water)
  3. Let the water incubate at room temperature for 10 minutes
  4. Take 1 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies

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