Team:Calgary/Notebook/Protocols/Process3
From 2011.igem.org
(Difference between revisions)
Emily Hicks (Talk | contribs) |
Emily Hicks (Talk | contribs) |
||
Line 7: | Line 7: | ||
<li type="1">Add the following to a 0.2mL thin-walled PCR tube sitting on ice:</li> | <li type="1">Add the following to a 0.2mL thin-walled PCR tube sitting on ice:</li> | ||
- | <table | + | <table border="1px"> |
- | + | ||
<tr> | <tr> | ||
<td><b>Component</b></td> | <td><b>Component</b></td> |
Revision as of 21:48, 27 September 2011
cDNA Synthesis/Reverse Transcription Reaction
Component | Volume |
RNA (40ng – 10ng to 1μg total RNA) | variable |
Nuclease free water | variable |
qScript cDNA synthesis mix | 4 uL |
Final Volume | 20 uL |