Team:Calgary/Notebook/Protocols/Process14

(Difference between revisions)

Revision as of 21:33, 27 September 2011

{{Team:Calgary/Notebookbar|

TITLE=Rehydration of Registry DNA| BODY=

Rehydration

Biobrick parts are shipped from the registry in a dehydrated from. As such they must be rehydrated before they can be used.

Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want
Add 10 μL of ddH20 (deionized water)
Let the water incubate at room temperature for 10 minutes
Take 1 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies

@@ Line 11: / Line 11: @@
      <tr>
        <td width="85%"><div class="heading"><a style="text-decoration:none" name="rehydration"></a> Rehydration</div></td>
-      <td width="15%"><a style="float:right;" href="#top" title="return to top">return to top</a> </td>
      </tr>
    </table>
@@ Line 17: / Line 16: @@
    <ol>
      <li> Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want</li>
-     <li> Add 15 μL of diH20 (deionized water)</li>
+     <li> Add 10 μL of ddH20 (deionized water)</li>
-     <li> Let the water sit for 5 minutes</li>
+     <li> Let the water incubate at room temperature for 10 minutes</li>
-     <li>Take 2 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies</li>
+     <li>Take 1 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies</li>
    </ol>
 </div>