Team:Calgary/Notebook/Protocols/Process2

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TITLE=RNA Extraction|
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TITLE=RNA Extraction from <i>Pseudomonas spp.</i>|
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<br>
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<h4> Reagents </h4>
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<ol>
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<li>TE buffer (10 mMTris•Cl, 1 mM EDTA, pH 8.0)containing 1 mg/ml lysozyme</li>
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<li>Buffer RLT with beta-mercaptoethanol (10μL/1mL of RLT)</li>
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<li>Buffer RPE with 95% ethanol (4 volumes ethanol to 1 volume ethanol)</li>
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<li>RNAprotect Bacterial Reagent </li>
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<li>95% ethanol</li>
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<li>RNeasy Mini Columns</li>
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<li>Genome Trap Columns</li>
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<li>Buffer RW1</li>
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<li>Buffer RDD</li>
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<li>DNase I</li>
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<li>RNAse Free Water</li>
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</ol>
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<br>
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<h4> Procedure </h4>

Revision as of 16:35, 27 September 2011

{{Team:Calgary/Notebookbar|

TITLE=RNA Extraction from Pseudomonas spp.| BODY=

Reagents

  1. TE buffer (10 mMTris•Cl, 1 mM EDTA, pH 8.0)containing 1 mg/ml lysozyme
  2. Buffer RLT with beta-mercaptoethanol (10μL/1mL of RLT)
  3. Buffer RPE with 95% ethanol (4 volumes ethanol to 1 volume ethanol)
  4. RNAprotect Bacterial Reagent
  5. 95% ethanol
  6. RNeasy Mini Columns
  7. Genome Trap Columns
  8. Buffer RW1
  9. Buffer RDD
  10. DNase I
  11. RNAse Free Water

Procedure

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