"
Team:UEA-JIC Norwich/Notebook
From 2011.igem.org
Benjevans1 (Talk | contribs) |
Benjevans1 (Talk | contribs) |
||
| Line 52: | Line 52: | ||
<body> | <body> | ||
<h1>Lab Journal</h1> | <h1>Lab Journal</h1> | ||
| - | <p>Monday 20th June - Attempted transformations of E.coli with biobricks for the first time. We used the Banana odour generator; red and green luciferase; and Epic biobricks. We followed the protocol offered by the New England Biolabs for this procedure (see methods). We find out tomorrow if it worked!</P> | + | <p><strong>Monday 20th June</strong> - Attempted transformations of E.coli with biobricks for the first time. We used the Banana odour generator; red and green luciferase; and Epic biobricks. We followed the protocol offered by the New England Biolabs for this procedure (see methods). We find out tomorrow if it worked!</P> |
| - | <p> | + | <p><strong>21st June</strong>- The control cells and 'Epic' Biobrick cells were successfully transformed. The cells transformed with the 'Banana odour' Biobrick mysteriously vanished from the incubator and were found a day later in the chill room. All other transformations failed. We repeated protocols of all unsuccessful transformations.</p> |
| - | <p> | + | <p><strong>22nd June</strong>- All transformations successful. Much relief. Made cultures from colonies of all transformations with the aim of making glycerol stock solutions as soon as possible.</P> |
<h1>Standard Journal</h1> | <h1>Standard Journal</h1> | ||
</body> | </body> | ||
</html> | </html> | ||
Revision as of 08:43, 23 June 2011
- Home
- The Team
- Aims
- Parts Submitted To The Registry
- Methods
- Lab Journal
- Lab Safety
- Follow Us
- Outreach
Lab Journal
Monday 20th June - Attempted transformations of E.coli with biobricks for the first time. We used the Banana odour generator; red and green luciferase; and Epic biobricks. We followed the protocol offered by the New England Biolabs for this procedure (see methods). We find out tomorrow if it worked!
21st June- The control cells and 'Epic' Biobrick cells were successfully transformed. The cells transformed with the 'Banana odour' Biobrick mysteriously vanished from the incubator and were found a day later in the chill room. All other transformations failed. We repeated protocols of all unsuccessful transformations.
22nd June- All transformations successful. Much relief. Made cultures from colonies of all transformations with the aim of making glycerol stock solutions as soon as possible.
