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Team:Caltech/Week 2
From 2011.igem.org
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== June 21 == | == June 21 == | ||
<p>MoBio kit extraction of DNA from collected samples<br/> | <p>MoBio kit extraction of DNA from collected samples<br/> | ||
| - | Enrichment cultures of samples in minimal media with 17α-estradiol, nonylphenol, and DDT</p> | + | Enrichment cultures of samples in minimal media with 17α-estradiol, nonylphenol, and DDT<br/> |
| + | Geneious and primer design tutorial</p> | ||
| + | |||
| + | == June 22 == | ||
| + | <p>Miniprep and sequencing of selected BioBricks (K123000, K123001, K123002, K123003) <br/> | ||
| + | Preparation of competent cells</p> | ||
| + | |||
| + | ==June 23== | ||
| + | <p>PCR,digest, ligation and transformation of human estrogen receptor BioBrick to remove intron<br/> | ||
| + | Start cultures of RFP, transformed HER<br/> | ||
| + | Design and order primers for the test sequence </p> | ||
}} | }} | ||
Revision as of 20:52, 22 June 2011
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Project |
June 19Collection of soil and water samples from LA River June 20Lab walkthrough June 21MoBio kit extraction of DNA from collected samples June 22Miniprep and sequencing of selected BioBricks (K123000, K123001, K123002, K123003) June 23PCR,digest, ligation and transformation of human estrogen receptor BioBrick to remove intron
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