"
Team:Northwestern/Notebook/Week11
From 2011.igem.org
(Difference between revisions)
| (3 intermediate revisions not shown) | |||
| Line 4: | Line 4: | ||
<html> | <html> | ||
<div id="header" style="margin: 14px 0px 0px 0px;"> | <div id="header" style="margin: 14px 0px 0px 0px;"> | ||
| - | <img src="https://static.igem.org/mediawiki/2011/4/40/Notebook_banner4_2.gif" height = " | + | <img src="https://static.igem.org/mediawiki/2011/4/40/Notebook_banner4_2.gif" height = "70px" width="750px" style="opacity:1;filter:alpha(opacity=100)" alt="NU-igem banner"/ border="0"> |
<div style="margin: -55px 0px 0px 80px;font:35px helvetica; color:#ffffff;"> | <div style="margin: -55px 0px 0px 80px;font:35px helvetica; color:#ffffff;"> | ||
Notebook <div style="margin: -40px 0px 0px 420px;font:35px helvetica; color:#444444;"> Week 11</div> | Notebook <div style="margin: -40px 0px 0px 420px;font:35px helvetica; color:#444444;"> Week 11</div> | ||
| Line 21: | Line 21: | ||
*Plated our glycerol stocks of the successful gels from friday to make sure everything worked properly. | *Plated our glycerol stocks of the successful gels from friday to make sure everything worked properly. | ||
*Ran a plate of our controls at the high-throughput lab. | *Ran a plate of our controls at the high-throughput lab. | ||
| - | *Digested and ran the genomic promoter ligations on | + | *Digested and ran the genomic promoter ligations on a gel |
<!-- ------------------------------------------------------------------- --> | <!-- ------------------------------------------------------------------- --> | ||
| Line 29: | Line 29: | ||
<div style="margin: -40px 0px 18px 20px;font:24px helvetica;font-weight:550; color:#ffffff;">Day 50 - Tuesday, August 23nd 2011 | <div style="margin: -40px 0px 18px 20px;font:24px helvetica;font-weight:550; color:#ffffff;">Day 50 - Tuesday, August 23nd 2011 | ||
</div></div></html> | </div></div></html> | ||
| - | * | + | *Analyzed the results from our control plate at the high throughput lab. |
| + | *We wanted to continue testing, but we were having trouble getting all of our samples to grow properly. | ||
| + | *Prepared 384 well plate layouts for future tests. | ||
<!-- ------------------------------------------------------------------- --> | <!-- ------------------------------------------------------------------- --> | ||
| Line 37: | Line 39: | ||
<div style="margin: -40px 0px 18px 20px;font:24px helvetica;font-weight:550; color:#ffffff;">Day 51 - Wednesday, August 24nd 2011 | <div style="margin: -40px 0px 18px 20px;font:24px helvetica;font-weight:550; color:#ffffff;">Day 51 - Wednesday, August 24nd 2011 | ||
</div></div></html> | </div></div></html> | ||
| - | *bacteria | + | *The bacteria continued to have very weird growth patterns. We tried two other spectrophotometers to try and judge if ours was malfunctioning. |
| + | *We eventually decided that our machine was okay, but that we had not been mixing the samples properly before reading them, which led to our weird results. | ||
| + | *We made new M9 media in case that was causing the growth problems. | ||
<!-- ------------------------------------------------------------------- --> | <!-- ------------------------------------------------------------------- --> | ||
| Line 43: | Line 47: | ||
<!-- ---------------------Day 52 --------------------------------------- --> | <!-- ---------------------Day 52 --------------------------------------- --> | ||
<html><div id="day47" style="margin: 0px 0px 0px 0px;"><img src="https://static.igem.org/mediawiki/2011/6/6b/Day_banner3.gif" height = "55px" width="450px" style="opacity:1;filter:alpha(opacity=100)" alt="day banner"/ border="0"> | <html><div id="day47" style="margin: 0px 0px 0px 0px;"><img src="https://static.igem.org/mediawiki/2011/6/6b/Day_banner3.gif" height = "55px" width="450px" style="opacity:1;filter:alpha(opacity=100)" alt="day banner"/ border="0"> | ||
| - | <div style="margin: -40px 0px 18px 20px;font:24px helvetica;font-weight:550; color:#ffffff;">Day | + | <div style="margin: -40px 0px 18px 20px;font:24px helvetica;font-weight:550; color:#ffffff;">Day 52 - Thursday, August 25nd 2011 |
</div></div></html> | </div></div></html> | ||
| - | * | + | *Ran a 96 well plate with our controls and GFP samples. We added the autoinducer to all of the samples at an OD of approximately 0.3 |
| + | *Began setting up interviews and a script for our human practices video | ||
<!-- ------------------------------------------------------------------- --> | <!-- ------------------------------------------------------------------- --> | ||
| Line 51: | Line 56: | ||
<!-- ---------------------Day 53 --------------------------------------- --> | <!-- ---------------------Day 53 --------------------------------------- --> | ||
<html><div id="day48" style="margin: 0px 0px 0px 0px;"><img src="https://static.igem.org/mediawiki/2011/6/6b/Day_banner3.gif" height = "55px" width="450px" style="opacity:1;filter:alpha(opacity=100)" alt="day banner"/ border="0"> | <html><div id="day48" style="margin: 0px 0px 0px 0px;"><img src="https://static.igem.org/mediawiki/2011/6/6b/Day_banner3.gif" height = "55px" width="450px" style="opacity:1;filter:alpha(opacity=100)" alt="day banner"/ border="0"> | ||
| - | <div style="margin: -40px 0px 18px 20px;font:24px helvetica;font-weight:550; color:#ffffff;">Day | + | <div style="margin: -40px 0px 18px 20px;font:24px helvetica;font-weight:550; color:#ffffff;">Day 53 - Friday, August 26nd 2011 |
</div></div></html> | </div></div></html> | ||
| - | * | + | *Analyzed the results from our GFP test. |
| + | *Continued working on human practices | ||
Latest revision as of 18:50, 25 September 2011
PROJECT
RESULTS
CONSIDERATIONS
ABOUT US
NOTEBOOK
ATTRIBUTIONS
Notebook
Week 11
Day 49 - Monday, August 22nd 2011
- More extensive minipreps - this time of all the genomic promoter ligations.
- Plated our glycerol stocks of the successful gels from friday to make sure everything worked properly.
- Ran a plate of our controls at the high-throughput lab.
- Digested and ran the genomic promoter ligations on a gel
Day 50 - Tuesday, August 23nd 2011
- Analyzed the results from our control plate at the high throughput lab.
- We wanted to continue testing, but we were having trouble getting all of our samples to grow properly.
- Prepared 384 well plate layouts for future tests.
Day 51 - Wednesday, August 24nd 2011
- The bacteria continued to have very weird growth patterns. We tried two other spectrophotometers to try and judge if ours was malfunctioning.
- We eventually decided that our machine was okay, but that we had not been mixing the samples properly before reading them, which led to our weird results.
- We made new M9 media in case that was causing the growth problems.
Day 52 - Thursday, August 25nd 2011
- Ran a 96 well plate with our controls and GFP samples. We added the autoinducer to all of the samples at an OD of approximately 0.3
- Began setting up interviews and a script for our human practices video
Day 53 - Friday, August 26nd 2011
- Analyzed the results from our GFP test.
- Continued working on human practices
