Team:Northwestern/Notebook/Week11

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<img src="https://static.igem.org/mediawiki/2011/4/40/Notebook_banner4_2.gif" height = "70px" width="750px" style="opacity:1;filter:alpha(opacity=100)" alt="NU-igem banner"/ border="0">
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Notebook  <div style="margin: -40px 0px 0px 420px;font:35px helvetica; color:#444444;">    &nbsp; Week 11</div>
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<!--Week 11-->
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<!-- ---------------------Day 49 --------------------------------------- -->
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<html><div id="day44" style="margin: 0px 0px 0px 0px;"><img src="https://static.igem.org/mediawiki/2011/6/6b/Day_banner3.gif" height = "55px" width="450px" style="opacity:1;filter:alpha(opacity=100)" alt="day banner"/ border="0">
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<div style="margin: -40px 0px 18px 20px;font:24px helvetica;font-weight:550; color:#ffffff;">Day 49 - Monday, August 22nd 2011
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*More extensive minipreps - this time of all the genomic promoter ligations.
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*Plated our glycerol stocks of the successful gels from friday to make sure everything worked properly.
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*Ran a plate of our controls at the high-throughput lab.
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*Digested and ran the genomic promoter ligations on a gel
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<html><div id="day45" style="margin: 0px 0px 0px 0px;"><img src="https://static.igem.org/mediawiki/2011/6/6b/Day_banner3.gif" height = "55px" width="450px" style="opacity:1;filter:alpha(opacity=100)" alt="day banner"/ border="0">
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<div style="margin: -40px 0px 18px 20px;font:24px helvetica;font-weight:550; color:#ffffff;">Day 50 - Tuesday, August 23nd 2011
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*Analyzed the results from our control plate at the high throughput lab.
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*We wanted to continue testing, but we were having trouble getting all of our samples to grow properly.
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*Prepared 384 well plate layouts for future tests.
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<html><div id="day46" style="margin: 0px 0px 0px 0px;"><img src="https://static.igem.org/mediawiki/2011/6/6b/Day_banner3.gif" height = "55px" width="470px" style="opacity:1;filter:alpha(opacity=100)" alt="day banner"/ border="0">
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<div style="margin: -40px 0px 18px 20px;font:24px helvetica;font-weight:550; color:#ffffff;">Day 51 - Wednesday, August 24nd 2011
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*The bacteria continued to have very weird growth patterns. We tried two other spectrophotometers to try and judge if ours was malfunctioning.
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*We eventually decided that our machine was okay, but that we had not been mixing the samples properly before reading them, which led to our weird results. 
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*We made new M9 media in case that was causing the growth problems.
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<html><div id="day47" style="margin: 0px 0px 0px 0px;"><img src="https://static.igem.org/mediawiki/2011/6/6b/Day_banner3.gif" height = "55px" width="450px" style="opacity:1;filter:alpha(opacity=100)" alt="day banner"/ border="0">
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<div style="margin: -40px 0px 18px 20px;font:24px helvetica;font-weight:550; color:#ffffff;">Day 52 - Thursday, August 25nd 2011
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*Ran a 96 well plate with our controls and GFP samples. We added the autoinducer to all of the samples at an OD of approximately 0.3
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*Began setting up interviews and a script for our human practices video
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<html><div id="day48" style="margin: 0px 0px 0px 0px;"><img src="https://static.igem.org/mediawiki/2011/6/6b/Day_banner3.gif" height = "55px" width="450px" style="opacity:1;filter:alpha(opacity=100)" alt="day banner"/ border="0">
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<div style="margin: -40px 0px 18px 20px;font:24px helvetica;font-weight:550; color:#ffffff;">Day 53 - Friday, August 26nd 2011
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*Analyzed the results from our GFP test.
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*Continued working on human practices

Latest revision as of 18:50, 25 September 2011

RETURN TO IGEM 2010



day banner
Day 49 - Monday, August 22nd 2011

  • More extensive minipreps - this time of all the genomic promoter ligations.
  • Plated our glycerol stocks of the successful gels from friday to make sure everything worked properly.
  • Ran a plate of our controls at the high-throughput lab.
  • Digested and ran the genomic promoter ligations on a gel


day banner
Day 50 - Tuesday, August 23nd 2011

  • Analyzed the results from our control plate at the high throughput lab.
  • We wanted to continue testing, but we were having trouble getting all of our samples to grow properly.
  • Prepared 384 well plate layouts for future tests.


day banner
Day 51 - Wednesday, August 24nd 2011

  • The bacteria continued to have very weird growth patterns. We tried two other spectrophotometers to try and judge if ours was malfunctioning.
  • We eventually decided that our machine was okay, but that we had not been mixing the samples properly before reading them, which led to our weird results.
  • We made new M9 media in case that was causing the growth problems.


day banner
Day 52 - Thursday, August 25nd 2011

  • Ran a 96 well plate with our controls and GFP samples. We added the autoinducer to all of the samples at an OD of approximately 0.3
  • Began setting up interviews and a script for our human practices video


day banner
Day 53 - Friday, August 26nd 2011

  • Analyzed the results from our GFP test.
  • Continued working on human practices
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