Team:UC Davis/Data LambdacI

From 2011.igem.org

(Difference between revisions)
Line 60: Line 60:
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
<span class="ydata">
<span class="ydata">
-
   0.220505,  
+
   0.515178,  
-
   0.041865,   
+
   0.148993,   
-
   0.037100,  
+
   0.116731,  
-
   0.036120,   
+
   0.092202,   
-
   0.035185,  
+
   0.086236,  
-
   0.034249,   
+
   0.085269,   
-
   0.032601,   
+
   0.081236,   
</span>
</span>
<h3>Mutant 3</h3>
<h3>Mutant 3</h3>
Line 84: Line 84:
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
  <span class="ydata">
  <span class="ydata">
-
   0.31888,  
+
   0.220505,  
-
   0.16625,   
+
   0.041865,   
-
   0.10228,  
+
   0.037100,  
-
   0.05821,   
+
   0.036120,   
-
   0.03985,  
+
   0.035185,  
-
   0.02549,   
+
   0.034249,   
-
   0.01329,  
+
   0.032601,  
</span>
</span>
<h3>Mutant 5</h3>
<h3>Mutant 5</h3>
Line 96: Line 96:
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
  <span class="ydata">
  <span class="ydata">
-
   0.63174,  
+
   0.919031,  
-
   0.33524,   
+
   0.234935,   
-
   0.17440,  
+
   0.180555,  
-
   0.09337,   
+
   0.162829,   
-
   0.03043,  
+
   0.157128,  
-
   0.03337,   
+
   0.152808,   
-
   0.01686,   
+
   0.141317,   
</span>
</span>
<h3>Mutant 6</h3>
<h3>Mutant 6</h3>
Line 108: Line 108:
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
  <span class="ydata">
  <span class="ydata">
-
   0.42290,  
+
   0.986299,  
-
   0.29347,   
+
   1.042648,   
-
   0.15733,  
+
   1.069110,  
-
   0.09471,   
+
   1.085407,   
-
   0.05778,  
+
   1.116565,  
-
   0.03850,   
+
   1.116565,   
-
   0.01475,   
+
   1.070768,   
</span>
</span>
<h3>Mutant 7</h3>
<h3>Mutant 7</h3>
Line 120: Line 120:
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
  <span class="ydata">
  <span class="ydata">
-
   0.58989,  
+
   0.470486,  
-
   0.32402,   
+
   0.131343,   
-
   0.20047,  
+
   0.104605,  
-
   0.11183,   
+
   0.081761,   
-
   0.06365,  
+
   0.075629,  
-
   0.03915,   
+
   0.067232,   
-
   0.01712,   
+
   0.062205, 
 +
</span>
 +
        <h3>Mutant 8</h3>
 +
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattagtgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgtagtgtggaattgtgagcggatatcaatttcacaca</span>
 +
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 +
<span class="ydata">
 +
  0.388973,
 +
  0.108555, 
 +
  0.086125,
 +
  0.075740, 
 +
  0.065796,
 +
  0.059857, 
 +
  0.053090,   
</span>
</span>
</div>
</div>

Revision as of 04:50, 25 September 2011

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Criteria

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λ cI Promoter Characterization Data

Wildtype

SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 1.010689, 0.967933, 0.880328, 0.734557, 0.662629, 0.607491, 0.530352, 4

Mutant 1

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.651227, 0.207767, 0.171959, 0.165190, 0.160246, 0.157574, 0.155748,

Mutant 2

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattattgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.515178, 0.148993, 0.116731, 0.092202, 0.086236, 0.085269, 0.081236,

Mutant 3

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaaagtgagttagctcactcattaggcaccccaggctttacactttaagcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.919031, 0.234935, 0.180555, 0.162829, 0.157128, 0.152808, 0.141317,

Mutant 4

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgcgcgcaacgcaactaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.220505, 0.041865, 0.037100, 0.036120, 0.035185, 0.034249, 0.032601,

Mutant 5

caatacgcaaaccgcctctccccgcgcgtcggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctccctcatcaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.919031, 0.234935, 0.180555, 0.162829, 0.157128, 0.152808, 0.141317,

Mutant 6

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctctacactttatgcttccggcttgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.986299, 1.042648, 1.069110, 1.085407, 1.116565, 1.116565, 1.070768,

Mutant 7

caatacgcaaaccgcctctccccgcgcgttggccgattcattagtgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgtagtgtggaattgtgagcggatatcaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.470486, 0.131343, 0.104605, 0.081761, 0.075629, 0.067232, 0.062205,

Mutant 8

caatacgcaaaccgcctctccccgcgcgttggccgattcattagtgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgtagtgtggaattgtgagcggatatcaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.388973, 0.108555, 0.086125, 0.075740, 0.065796, 0.059857, 0.053090,
Some preliminary data. These are LacI promoter mutants. The green bars represent mutants that are outside of 1.5 standard deviations of wildtype LacI promoter. There's still more to come!

Retrieved from "http://2011.igem.org/Team:UC_Davis/Data_LambdacI"