Team:UC Davis/Data LambdacI

From 2011.igem.org

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<h1 id="widget">LacI Promoter Characterization Data</h1>
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<div id="mutantwidget" class="floatbox2">
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<h3>Wildtype</h3>
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<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
 +
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata">
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0.99315,
 +
  0.78019, 
 +
  0.58405,
 +
  0.45400, 
 +
  0.35401,
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  0.29099, 
 +
  0.13305, 
 +
</span>
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<span class="numconditions">4</span>
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<span class='xdata_3d'>0, 1, 2, 5</span>
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<span class='ydata_3d'>0.00000,  0.20000,  0.40000,  0.60000,  0.80000,  1.00000,  2.00000</span>
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<span class='zdata_3d'>
 +
  0.99315,  1.02097,  0.99023,  0.99354,
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  0.78019,  0.97021,  0.96725,  0.96226,
 +
  0.58405,  0.87100,  0.89795,  0.94529,
 +
  0.45400,  0.76333,  0.85071,  0.93618,
 +
  0.35401,  0.65243,  0.75444,  0.88056,
 +
  0.29099,  0.55454,  0.68266,  0.84441,
 +
  0.13305,  0.26829,  0.37015,  0.55294</span>
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<h3>Mutant 1</h3>
 +
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
 +
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata">
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  0.68829,
 +
  0.48655, 
 +
  0.27198,
 +
  0.15260, 
 +
  0.08426,
 +
  0.05322, 
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  0.01741, 
 +
</span>
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<h3>Mutant 2</h3>
 +
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattattgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca</span>
 +
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata">
 +
  0.68514,
 +
  0.43727, 
 +
  0.27724,
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  0.17817, 
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  0.11684,
 +
  0.08021, 
 +
  0.03415, 
 +
</span>
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<h3>Mutant 3</h3>
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<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaaagtgagttagctcactcattaggcaccccaggctttacactttaagcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
 +
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata">
 +
  0.67026,
 +
  0.44322, 
 +
  0.28504,
 +
  0.17543, 
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  0.10938,
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  0.07325, 
 +
  0.02937, 
 +
</span>
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<h3>Mutant 4</h3>
 +
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgcgcgcaacgcaactaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca</span>
 +
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 +
<span class="ydata">
 +
  0.31888,
 +
  0.16625, 
 +
  0.10228,
 +
  0.05821, 
 +
  0.03985,
 +
  0.02549, 
 +
  0.01329, 
 +
</span>
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<h3>Mutant 5</h3>
 +
<span class="sequence">caatacgcaaaccgcctctccccgcgcgtcggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctccctcatcaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
 +
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata">
 +
  0.63174,
 +
  0.33524, 
 +
  0.17440,
 +
  0.09337, 
 +
  0.03043,
 +
  0.03337, 
 +
  0.01686, 
 +
</span>
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<h3>Mutant 6</h3>
 +
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctctacactttatgcttccggcttgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
 +
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 +
<span class="ydata">
 +
  0.42290,
 +
  0.29347, 
 +
  0.15733,
 +
  0.09471, 
 +
  0.05778,
 +
  0.03850, 
 +
  0.01475, 
 +
</span>
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<h3>Mutant 7</h3>
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<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattagtgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgtagtgtggaattgtgagcggatatcaatttcacaca</span>
 +
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 +
<span class="ydata">
 +
  0.58989,
 +
  0.32402, 
 +
  0.20047,
 +
  0.11183, 
 +
  0.06365,
 +
  0.03915, 
 +
  0.01712, 
 +
</span>
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</div>
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</div>
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<div class="floatbox">
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<center>
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<div class="floatbox3">
<img src="https://static.igem.org/mediawiki/2011/d/d9/UCD_Mut_graph_lacI.png" width="680" height="350">
<img src="https://static.igem.org/mediawiki/2011/d/d9/UCD_Mut_graph_lacI.png" width="680" height="350">
Some preliminary data.  These are LacI promoter mutants.  The green bars represent mutants that are outside of 1.5 standard deviations of wildtype LacI promoter.  There's still more to come!
Some preliminary data.  These are LacI promoter mutants.  The green bars represent mutants that are outside of 1.5 standard deviations of wildtype LacI promoter.  There's still more to come!
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Revision as of 04:07, 25 September 2011

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Criteria

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LacI Promoter Characterization Data

Wildtype

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.99315, 0.78019, 0.58405, 0.45400, 0.35401, 0.29099, 0.13305, 4 0, 1, 2, 5 0.00000, 0.20000, 0.40000, 0.60000, 0.80000, 1.00000, 2.00000 0.99315, 1.02097, 0.99023, 0.99354, 0.78019, 0.97021, 0.96725, 0.96226, 0.58405, 0.87100, 0.89795, 0.94529, 0.45400, 0.76333, 0.85071, 0.93618, 0.35401, 0.65243, 0.75444, 0.88056, 0.29099, 0.55454, 0.68266, 0.84441, 0.13305, 0.26829, 0.37015, 0.55294

Mutant 1

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.68829, 0.48655, 0.27198, 0.15260, 0.08426, 0.05322, 0.01741,

Mutant 2

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattattgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.68514, 0.43727, 0.27724, 0.17817, 0.11684, 0.08021, 0.03415,

Mutant 3

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaaagtgagttagctcactcattaggcaccccaggctttacactttaagcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.67026, 0.44322, 0.28504, 0.17543, 0.10938, 0.07325, 0.02937,

Mutant 4

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgcgcgcaacgcaactaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.31888, 0.16625, 0.10228, 0.05821, 0.03985, 0.02549, 0.01329,

Mutant 5

caatacgcaaaccgcctctccccgcgcgtcggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctccctcatcaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.63174, 0.33524, 0.17440, 0.09337, 0.03043, 0.03337, 0.01686,

Mutant 6

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctctacactttatgcttccggcttgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.42290, 0.29347, 0.15733, 0.09471, 0.05778, 0.03850, 0.01475,

Mutant 7

caatacgcaaaccgcctctccccgcgcgttggccgattcattagtgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgtagtgtggaattgtgagcggatatcaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.58989, 0.32402, 0.20047, 0.11183, 0.06365, 0.03915, 0.01712,
Some preliminary data. These are LacI promoter mutants. The green bars represent mutants that are outside of 1.5 standard deviations of wildtype LacI promoter. There's still more to come!

Retrieved from "http://2011.igem.org/Team:UC_Davis/Data_LambdacI"