Team:Caltech/Recipes

From 2011.igem.org

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'''Agar/LB plate (Autoclaved):'''  
'''Agar/LB plate (Autoclaved):'''  
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* 500 g of nanopure water
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* 500 mL nanopure water
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* 10 g of Tryptone
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* 10 g tryptone
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* 10 g of NaCl  
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* 10 g NaCl  
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* 5 g of yeast extract
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* 5 g yeast extract
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* 15 g of Agar
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* 15 g agar
<br/>  
<br/>  
-
'''50% Glycerol Stock:'''  
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'''50% glycerol Stock:'''  
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* 50 mL of glycerol  
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* 50 mL glycerol  
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* 50 mL of nanopure water  
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* 50 mL nanopure water  
<br/>  
<br/>  
<p>'''Enrichment Minimal Media'''</p>   
<p>'''Enrichment Minimal Media'''</p>   
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* 1.0712 g of K2 HPO4
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* 1.0712 g K2 HPO4
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* 0.5239 g of KH2 PO4
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* 0.5239 g KH2 PO4
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* 79 mL of Phosphate solution  
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* 79 mL Phosphate solution  
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* 100 mL of Salt Solution I  
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* 100 mL Salt Solution I  
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* 100 mL of Salt Solution II  
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* 100 mL Salt Solution II  
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* 10 mL of Wolfe's Vitamin Solution  
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* 10 mL Wolfe's Vitamin Solution  
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* 1 mL of SL-10 Trace Element Solution.
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* 1 mL SL-10 Trace Element Solution.
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and then add 5 mg of whatever chemical used in two flasks, one with vitamins and one without.
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* variable amounts of EDC's}}
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}}
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Revision as of 22:56, 21 June 2011


Caltech iGEM 2011



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Agar/LB plate (Autoclaved):
  • 500 mL nanopure water
  • 10 g tryptone
  • 10 g NaCl
  • 5 g yeast extract
  • 15 g agar


50% glycerol Stock:

  • 50 mL glycerol
  • 50 mL nanopure water


Enrichment Minimal Media

  • 1.0712 g K2 HPO4
  • 0.5239 g KH2 PO4
  • 79 mL Phosphate solution
  • 100 mL Salt Solution I
  • 100 mL Salt Solution II
  • 10 mL Wolfe's Vitamin Solution
  • 1 mL SL-10 Trace Element Solution.
  • variable amounts of EDC's


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