"
Team:UC Davis/Notebook/Week 14
From 2011.igem.org
| Line 34: | Line 34: | ||
--Wednesday 9/14/11-- | --Wednesday 9/14/11-- | ||
| - | Last night we did more of the extra-characterizing runs for LacI mutants #5 (K61105) and today just put one for #6 (K611026) in the Tecan. Unfortunately, it seems that something has gone wrong with our data past few runs. The 0% arabinose level for all of our runs looks good, but everything from 0.2%-2% arabinose seems to fully repress our mutant promoters. Considering that the new LB+arabinose stocks that we made yesterday was carefully measured, there's a good probability that the old stock we were using (which was made before the summer) was mislabeled. Darn, just when things started getting on the right track again! It seems as though we're going to take a minor halt in our characterizing runs in order to calibrate our old data. | + | Last night we did more of the extra-characterizing runs for LacI mutants #5 (K61105) and today just put one for #6 (K611026) in the Tecan. Unfortunately, it seems that something has gone wrong with our data the past few runs. The 0% arabinose level for all of our runs looks good, but everything from 0.2%-2% arabinose seems to fully repress our mutant promoters. Considering that the new LB+arabinose stocks that we made yesterday was carefully measured, there's a good probability that the old stock we were using (which was made before the summer) was mislabeled. Darn, just when things started getting on the right track again! It seems as though we're going to take a minor halt in our characterizing runs in order to calibrate our old data. |
| + | |||
| + | <html></div><div class="floatbox"></html> | ||
| + | |||
| + | --Thursday 9/15/11-- | ||
| + | |||
| + | In order to calibrate our old arabinose measurements, we're going to do serial dilutions of our new LB+chloro+arabinose stocks with known arabinose concentrations. We're starting with 0.2% arabinose and halving the concentration in each consecutive well. Each well will be inoculated with our the LacI wildtype construct, since we probably have the best understanding/characterization of this part so far. While analyzing this data we'll also do more runs with our R0040 and R0051 mutants. | ||
| + | |||
| + | <html></div><div class="floatbox"></html> | ||
| + | |||
| + | --Friday 9/16/11-- | ||
| + | |||
| + | |||
| + | |||
<html></div></html> | <html></div></html> | ||
Revision as of 18:14, 24 September 2011
Our Sponsors
Start a Family
Got a favorite BioBrick? Check our our process for expanding basic parts into part families.Criteria
View our judging criteria for iGEM 2011 here.
Week 14
--Monday 9/12/11--
Today, we all regrouped to work out the plan for the week. Our primary concern now is to get R0040 and R0051 mutants before the deadline. Most of the screening results don't look very good, but we think that we can see a few mutants from each set. We're going to do another screen to see if we can possibly get good data from another screen of both R0040 and R0051 mutants. Tonight, when that run is over, we'll do a 1 mutant, 7 arabinose level, 4 IPTG level run for LacI mutant #4 (K611024) just to keep the Tecan busy. Tomorrow we'll look at this screening data and hopefully see some good results!
--Tuesday 9/13/11--
Today we're still passing the time by doing the 1 mutant, 7 arabinose level, 4 IPTG level runs for LacI mutant #7 (K611027). Aside from the Tecan, we had a very productive day. We've made just about everything we could with LB and chloro: LB+chloro plates, LB+chloro media, and LB+chloro+arabinose stocks at many different levels of arabinose.
--Wednesday 9/14/11--
Last night we did more of the extra-characterizing runs for LacI mutants #5 (K61105) and today just put one for #6 (K611026) in the Tecan. Unfortunately, it seems that something has gone wrong with our data the past few runs. The 0% arabinose level for all of our runs looks good, but everything from 0.2%-2% arabinose seems to fully repress our mutant promoters. Considering that the new LB+arabinose stocks that we made yesterday was carefully measured, there's a good probability that the old stock we were using (which was made before the summer) was mislabeled. Darn, just when things started getting on the right track again! It seems as though we're going to take a minor halt in our characterizing runs in order to calibrate our old data.
--Thursday 9/15/11--
In order to calibrate our old arabinose measurements, we're going to do serial dilutions of our new LB+chloro+arabinose stocks with known arabinose concentrations. We're starting with 0.2% arabinose and halving the concentration in each consecutive well. Each well will be inoculated with our the LacI wildtype construct, since we probably have the best understanding/characterization of this part so far. While analyzing this data we'll also do more runs with our R0040 and R0051 mutants.
--Friday 9/16/11--
