Team:Caltech/Protocols
From 2011.igem.org
Line 2: | Line 2: | ||
Content= | Content= | ||
- | <p>'''Transforming DNA | + | <p>'''Transforming DNA:'''<br/> |
1) Thaw competent cells on ice: 15K, 15J, 15O, 15M.<br/> | 1) Thaw competent cells on ice: 15K, 15J, 15O, 15M.<br/> | ||
2) Get 10 micro Liters of pure water.<br/> | 2) Get 10 micro Liters of pure water.<br/> | ||
Line 15: | Line 15: | ||
(STILL CONFUSED ABOUT THIS PROTOCOL NOT SURE IF RIGHT)</p> | (STILL CONFUSED ABOUT THIS PROTOCOL NOT SURE IF RIGHT)</p> | ||
- | |||
- | |||
- | |||
- | |||
<p>'''I don't know what this is called'''...Putting 100 mg soil into each tube, then put the tubes in shakers overnight at 30°C and room temperature respectively???</p> | <p>'''I don't know what this is called'''...Putting 100 mg soil into each tube, then put the tubes in shakers overnight at 30°C and room temperature respectively???</p> | ||
Line 27: | Line 23: | ||
3) Put in some beads and shake gently.<br/> | 3) Put in some beads and shake gently.<br/> | ||
4) Put the dirty beads away in the dirty bead container.</p> | 4) Put the dirty beads away in the dirty bead container.</p> | ||
- | |||
- | |||
}} | }} | ||
Revision as of 22:32, 21 June 2011
Project |
Transforming DNA: 1) Thaw competent cells on ice: 15K, 15J, 15O, 15M.
I don't know what this is called...Putting 100 mg soil into each tube, then put the tubes in shakers overnight at 30°C and room temperature respectively??? Starter Competent Cell Culture Recipe?
|