From 2011.igem.org
(Difference between revisions)
|
|
| (3 intermediate revisions not shown) |
| Line 1: |
Line 1: |
| - | Spin 1.5 mL of overnight culture for 30 secs. in micro-centrifuge. Aspirate off all but 200uL of supernatant and resuspend the pellet by vortexing. Add 300uL of TENS and mix by inversion . The solution should become viscous add 150uL of sodium acetate and vortex. A fine white precipitate should form. Centrifuge for 2.5 minutes. Transfer the supernatant to clean tube and add 2 volumes (1mL) of room temp ETOH. Mix and pellet DNA by centrifugation for 2-5 min. Wash pellet with 70% ethanol and allow pellet to dry. Resuspend the pellet in 30uL TE. Digest 5-10uL as usual.
| + | <gallery widths=910px heights=800px> |
| - | :PNICE--5/12/11 | + | File:May26June6GDS.jpg |
| - | :PNICE (tKAN)--5/23/11
| + | </gallery> |
| - | :J04430--5/23/11
| + | |
| - | :J04430--5/12/11
| + | |
| - | :Trans 003-W1--5/12/11
| + | |
| - | :Trans 003-W1--5/23/11
| + | |
| - | | + | |
| - | P.P. G.E. S.D.
| + | |
Latest revision as of 15:11, 24 September 2011
"
