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Team:Kyoto/Protocol
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# After autoclave, add 10 ml filter sterilized 100 mM MgSO4, 20% glucose, 10 mM CaCl2, 100 mM thiamine-HCl. | # After autoclave, add 10 ml filter sterilized 100 mM MgSO4, 20% glucose, 10 mM CaCl2, 100 mM thiamine-HCl. | ||
# If you need, add 10ml filter sterilized 20% casamino acid. | # If you need, add 10ml filter sterilized 20% casamino acid. | ||
| + | |} | ||
| + | <br/> | ||
| + | ===SOB medium=== | ||
| + | ::{| class="wikitable" | ||
| + | ! width="300" |Materials | ||
| + | ! width="300" |Methods | ||
| + | |- | ||
| + | | | ||
| + | * water : 100 mL | ||
| + | * Tryptone: 2 g | ||
| + | * Yeast extract : 0.5 g | ||
| + | * 5M NaCl : 0.5 g | ||
| + | * 3M KCl : 1 g | ||
| + | * 10mM MgSO4 : 1 ml | ||
| + | * 10mM MgCl2 : 1 ml | ||
| + | | | ||
| + | # Stir Tryptone and Yeast extract with water. | ||
| + | # Add 200ul 5M NaCl and 84ul 3M KCl | ||
| + | # After autoclave, add 1 ml filter sterilized 10 mM MgSO4 and 10mM MgCl2. | ||
|} | |} | ||
</div> | </div> | ||
<!-- end of main --> | <!-- end of main --> | ||
Revision as of 05:18, 23 September 2011
Contents |
Protocol
Medium for drosophila
[http://www.biol.se.tmu.ac.jp/fly/www/standard-medium.html Medium for drosophila] Materials Methods - water : 500mL
- dry yeast : 20g
- corn flour : 45g
- glucose : 50g
- agarose : 3.5~5g
- propionic acid : 1.5mL
- 10% p-hydroxybenzoate in 70% Eternol : 5g
- Stir dry yeast and agarose with about two-thirds of water. Then, autoclave it.
- Stir corn flour and glucose with the remaining water.
- Stir 1 and 2, then autoclave it again.
- after autoclave, add propionic acid and 10% p-hydroxybenzoate in 70% Eternol into it.■
M9 medium
Materials Methods - water : 1 L
- Na2HPO4: 6 g
- KH2PO4 : 3 g
- NaCl : 0.5 g
- NH4Cl : 1 g
- 100 mM MgSO4 : 10 ml
- 20% glucose : 10 mL
- 10 mM CaCl2 : 10 ml
- 100 mM thiamine-HCl : 10 ml
- 20% casamino acid : 10 ml
- Stir Na2HPO4, KH2PO4, NaCl and NH4Cl with water.
- After autoclave, add 10 ml filter sterilized 100 mM MgSO4, 20% glucose, 10 mM CaCl2, 100 mM thiamine-HCl.
- If you need, add 10ml filter sterilized 20% casamino acid.
SOB medium
Materials Methods - water : 100 mL
- Tryptone: 2 g
- Yeast extract : 0.5 g
- 5M NaCl : 0.5 g
- 3M KCl : 1 g
- 10mM MgSO4 : 1 ml
- 10mM MgCl2 : 1 ml
- Stir Tryptone and Yeast extract with water.
- Add 200ul 5M NaCl and 84ul 3M KCl
- After autoclave, add 1 ml filter sterilized 10 mM MgSO4 and 10mM MgCl2.
