Team:Kyoto/Protocol

From 2011.igem.org

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(M9 medium)
(M9 medium)
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* 100 mM thiamine-HCl : 10 ml
* 100 mM thiamine-HCl : 10 ml
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# Stir dry yeast and agarose with about two-thirds of water. Then, autoclave it.
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# Stir Na2HPO4, KH2PO4, NaCl and NH4Cl with water.
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# Stir corn flour and glucose with the remaining water.
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# After autoclave, add 10 ml filter sterilized 100 mM MgSO4, 20% glucose, 10 mM CaCl2, 100 mM thiamine-HCl.
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# Stir 1 and 2, then autoclave it again.
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# after autoclave, add propionic acid and 10% p-hydroxybenzoate in 70% Eternol into it.
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Revision as of 04:59, 23 September 2011

Protocol

Medium for drosophila

[http://www.biol.se.tmu.ac.jp/fly/www/standard-medium.html Medium for drosophila]
Materials Methods
  • water : 500mL
  • dry yeast : 20g
  • corn flour : 45g
  • glucose : 50g
  • agarose : 3.5~5g
  • propionic acid : 1.5mL
  • 10% p-hydroxybenzoate in 70% Eternol : 5g
  1. Stir dry yeast and agarose with about two-thirds of water. Then, autoclave it.
  2. Stir corn flour and glucose with the remaining water.
  3. Stir 1 and 2, then autoclave it again.
  4. after autoclave, add propionic acid and 10% p-hydroxybenzoate in 70% Eternol into it.■


M9 medium

Materials Methods
  • water : 1 L
  • Na2HPO4: 6 g
  • KH2PO4 : 3 g
  • NaCl : 0.5 g
  • NH4Cl : 1 g
  • 100 mM MgSO4 : 10 ml
  • 20% glucose : 10 mL
  • 10 mM CaCl2 : 10 ml
  • 100 mM thiamine-HCl : 10 ml
  1. Stir Na2HPO4, KH2PO4, NaCl and NH4Cl with water.
  2. After autoclave, add 10 ml filter sterilized 100 mM MgSO4, 20% glucose, 10 mM CaCl2, 100 mM thiamine-HCl.