Team:Paris Bettencourt/Experiments/Methodologies/K090403J

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After having used a replicative plasmid (pHM3), we tried to use a Bacillus subtilis integration plasmid. For that, we used and plan to continue the construction of the B.subtilis integration vector pSBINT1 built by the 2008 Cambridge iGEM team.
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<h1>Integration plasmid</h1>
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In parallel to the transformation with the replicative plasmid (pHM3), we tried to use a Bacillus subtilis integration plasmid. For that, we used the construction of the B.subtilis integration vector pSBINT1 built by the 2008 Cambridge iGEM team.
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|+T7 autoloop RFP+ Control / E-coli at 37°C
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|[[File:PSBINT1_final.png|290px|thumb|center|E-Coli  at 37°C  (trans image)]]
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|[[File:PBSINT1_final.png|900px|thumb|center|]]
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<p>At first, we managed to clone each of our parts in the 5' integration sequence for the AmyE locus of B.subtilis (<partinfo>K143001</partinfo>).</p>
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<p>After, we want to clone them into the integrative plasmid (<partinfo>K090403</partinfo>).</p>
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Latest revision as of 02:34, 22 September 2011

Team IGEM Paris 2011

Integration plasmid

In parallel to the transformation with the replicative plasmid (pHM3), we tried to use a Bacillus subtilis integration plasmid. For that, we used the construction of the B.subtilis integration vector pSBINT1 built by the 2008 Cambridge iGEM team.

Bacillus subtilis integration vector
PBSINT1 final.png

At first, we managed to clone each of our parts in the 5' integration sequence for the AmyE locus of B.subtilis (<partinfo>K143001</partinfo>).

After, we want to clone them into the integrative plasmid (<partinfo>K090403</partinfo>).

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