Team:Glasgow/BiofilmResults
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<h1>Results</h1> | <h1>Results</h1> | ||
+ | <h6><a href="https://2011.igem.org/Team:Glasgow/Biofilm">Back to Biofilms</a></h6> | ||
+ | <h6><a href="https://2011.igem.org/Team:Glasgow/Results">Back to Results</a></h6> | ||
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<p>The images below show a selection of stages of biofilm formation. Starting with Image 1 showing a lab strain of <i>E.coli</i>that has no fimbriae, and is not forming a biofilm.</p> | <p>The images below show a selection of stages of biofilm formation. Starting with Image 1 showing a lab strain of <i>E.coli</i>that has no fimbriae, and is not forming a biofilm.</p> | ||
<p>Image 2 shows an EM of <i>E.coli</i> Nissle 1917 in the early stages of biofilm formation. The fimbriae that allow the cells to cling to each other are clearly visible.</p> | <p>Image 2 shows an EM of <i>E.coli</i> Nissle 1917 in the early stages of biofilm formation. The fimbriae that allow the cells to cling to each other are clearly visible.</p> |
Revision as of 02:33, 22 September 2011
Results
Back to Biofilms
Back to Results
The images below show a selection of stages of biofilm formation. Starting with Image 1 showing a lab strain of E.colithat has no fimbriae, and is not forming a biofilm.
Image 2 shows an EM of E.coli Nissle 1917 in the early stages of biofilm formation. The fimbriae that allow the cells to cling to each other are clearly visible.
Image 3 shows a Nissle biofilm in the later stages of formation, with the cells densely packed and the extracellular matrix that holds them together showing.
Image 1: 15,000x EM of E.coli for comparison. No fimbriae or EPS is visible. (courtesy of Rocky Mountain Laboratories) |
Image 2: 10,000x SEM image of Nissle showing the fimbriae |
Image 3: SEM image of Nissle biofilm showing the extracellular matrix |
Image 4: 1000x EM of P. aeruginosa biofilm, showing its densely packed structure (courtesy of Dan Walker, University of Glasgow) |
Figure 1: Comparison of RFP E.coli Nissle biofilms to untransformed E.coli Nissle bioflims under light microscope and under excitatory and non-excitatory wavelengths |
Figure 2: Comparison of RFP E.coli Nissle biofilms to puC19 E.coli Nissle biofilms under light microscope and under excitatory and non-excitatory wavelengths |
Summary
- New Chassis
-Transformable
- Forms biofilms
- Non-pathogenic and compatible with majority of biobricks
-No shuttle vector necessary
-Time series shows that biofilm grows at similar speed to planktonic cells
Well suited for biofilm investigation, especially when intending to transform the biofilm