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Team:Paris Bettencourt/Experiments/Methodologies/K090403J
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| - | At first, we managed to clone each of our parts in the 5' integration sequence for the AmyE locus of B.subtilis (K143001). After, we want to clone them into the integrative plasmid. | + | At first, we managed to clone each of our parts in the 5' integration sequence for the AmyE locus of B.subtilis (K143001). After, we want to clone them into the integrative plasmid (K090403). |
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Revision as of 01:50, 22 September 2011
Integration
After having used a replicative plasmid (pHM3), we tried to use a Bacillus subtilis integration plasmid. For that, we used and plan to continue the construction of the B.subtilis integration vector pSBINT1 built by the 2008 Cambridge iGEM team.
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At first, we managed to clone each of our parts in the 5' integration sequence for the AmyE locus of B.subtilis (K143001). After, we want to clone them into the integrative plasmid (K090403).
