Team:WarrenCIndpls IN-HS/Project
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=== The Experiment === | === The Experiment === | ||
- | '''DNA Extraction''' | + | '''DNA Extraction:''' |
+ | |||
1) Pipet 200 microliters of distilled water into two centrifuge tubes | 1) Pipet 200 microliters of distilled water into two centrifuge tubes | ||
+ | |||
2) Add a drop of detergent to each tube and shake. | 2) Add a drop of detergent to each tube and shake. | ||
+ | |||
3) Label the tubes according to what will go into each. | 3) Label the tubes according to what will go into each. | ||
- | 4) Rub the loop end of the inoculating loop across the surface of the agar with the E.Coli containing the ADH terminator. | + | |
+ | 4) Rub the loop end of the inoculating loop across the surface of the agar with the E.Coli containing the ADH | ||
+ | terminator. | ||
+ | |||
5) Place the loop into the corresponding tube and swivel the loop a few times. | 5) Place the loop into the corresponding tube and swivel the loop a few times. | ||
+ | |||
6) Repeat 4-5 with the E.Coli containing M-Cherry. | 6) Repeat 4-5 with the E.Coli containing M-Cherry. | ||
+ | |||
7) Shake the tubes well or place them on a vortex for about 5 seconds. | 7) Shake the tubes well or place them on a vortex for about 5 seconds. | ||
+ | |||
8) Add a pinch of meat tenderizer to each tube. | 8) Add a pinch of meat tenderizer to each tube. | ||
-this will break apart the proteins holding the cells together. | -this will break apart the proteins holding the cells together. | ||
+ | |||
9) Vortex the solutions again. | 9) Vortex the solutions again. | ||
+ | |||
10) Place them in a water bath at 56-60 degrees Celsius | 10) Place them in a water bath at 56-60 degrees Celsius | ||
'''DNA Amplification''' | '''DNA Amplification''' | ||
+ | |||
1) | 1) | ||
+ | |||
+ | 2) | ||
== Results == | == Results == |
Revision as of 16:08, 20 June 2011
Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
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Contents |
Overall project
Project Details
Gibson Assembly
The Experiment
DNA Extraction:
1) Pipet 200 microliters of distilled water into two centrifuge tubes
2) Add a drop of detergent to each tube and shake.
3) Label the tubes according to what will go into each.
4) Rub the loop end of the inoculating loop across the surface of the agar with the E.Coli containing the ADH terminator.
5) Place the loop into the corresponding tube and swivel the loop a few times.
6) Repeat 4-5 with the E.Coli containing M-Cherry.
7) Shake the tubes well or place them on a vortex for about 5 seconds.
8) Add a pinch of meat tenderizer to each tube. -this will break apart the proteins holding the cells together.
9) Vortex the solutions again.
10) Place them in a water bath at 56-60 degrees Celsius
DNA Amplification
1)
2)