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Team:Freiburg/Notebook/28 June
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| - | == | + | {{:Team:Freiburg/Templates/header}} |
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| + | <div id="notebook-page-header"> | ||
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| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/27_June">Previous entry</a> | ||
| + | </div> | ||
| + | <div id="notebook-title"> | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook"> 28 June </a> | ||
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| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/29_June">Next entry</a> | ||
| + | </div> | ||
| + | </div> | ||
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| - | ==3A assembly:Digestion, Ligation and Transformation for the receptor== | + | ==<span style="color:green;">green light receptor</span>== |
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| + | ===Digestion of minipreps for the green light receptor=== | ||
| + | '''Investigators: Sophie''' | ||
| + | |||
| + | Digestion of minipreps: | ||
| + | *S 17 (Ccar 1:1) | ||
| + | *S 18 (Ccar 1:3) | ||
| + | *S 19 (Ccas 1:1) | ||
| + | *S 20 (Ccas 1:3) | ||
| + | *S 21 (artificial terminator) | ||
| + | |||
| + | Enzyme 1: XbaI | ||
| + | Enzyme 2: PstI | ||
| + | |||
| + | two gels were loaded. | ||
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| + | <span style="color:blue;">Comments: S17, S18 and S20 were ok. S21 was not digested.</span> | ||
| + | |||
| + | ==<span style="color:red;">red light receptor</span>== | ||
| + | ===3A assembly:Digestion, Ligation and Transformation for the receptor=== | ||
'''Investigators: Julia''' | '''Investigators: Julia''' | ||
| - | Digestion of ho1, | + | Digestion of ho1, pcyA (chromophor) and terminator (pSB1AK3). |
| - | Ho1 and | + | Ho1 and pcyA:<br/> |
Enzyme 1: EcoRI<br/> | Enzyme 1: EcoRI<br/> | ||
Enzyme 2: SpeI<br/> | Enzyme 2: SpeI<br/> | ||
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ligated vector was then transformed in cells and plated out on chloramphenicol plates. | ligated vector was then transformed in cells and plated out on chloramphenicol plates. | ||
| + | <span style="color:blue;">Comments: The terminator between ho1 and pycA was missing. The digestion was repeated on june, 29.</span> | ||
| - | == | + | ==<span style="color:grey;">Precipitator</span>== |
| + | ===PCR of GFP fused to plastic-binding domain=== | ||
'''Investigators: Sophie, Rüdiger''' | '''Investigators: Sophie, Rüdiger''' | ||
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*GFPpbdSpedw (P3) | *GFPpbdSpedw (P3) | ||
| - | <span style="color:blue;">Comments: PCR did not work, because of too high concentration of primers</span> | + | <span style="color:blue;">Comments: PCR did not work, because of too high concentration of primers. PCR was repeated on june, 30</span> |
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Latest revision as of 01:23, 22 September 2011
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This is the wiki page of the Freiburger student team competing for iGEM 2011. Thank you for your interest!
