Team:Freiburg/Notebook/28 June

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=='''21. Labday'''==
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/27_June">Previous entry</a>
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<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 28 June </a>
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/29_June">Next entry</a>
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==3A assembly:Digestion, Ligation and Transformation for the receptor==
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==<span style="color:green;">green light receptor</span>==
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===Digestion of minipreps for the green light receptor===
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'''Investigators: Sophie'''
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Digestion of minipreps:
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*S 17 (Ccar 1:1)
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*S 18 (Ccar 1:3)
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*S 19 (Ccas 1:1)
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*S 20 (Ccas 1:3)
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*S 21 (artificial terminator)
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Enzyme 1: XbaI
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Enzyme 2: PstI
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two gels were loaded.
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<span style="color:blue;">Comments: S17, S18 and S20 were ok. S21 was not digested.</span>
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==<span style="color:red;">red light receptor</span>==
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===3A assembly:Digestion, Ligation and Transformation for the receptor===
'''Investigators: Julia'''
'''Investigators: Julia'''
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Digestion of ho1, pycA (chromophor) and terminator (pSB1AK3).  
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Digestion of ho1, pcyA (chromophor) and terminator (pSB1AK3).  
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Ho1 and pycA:<br/>
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Ho1 and pcyA:<br/>
Enzyme 1: EcoRI<br/>
Enzyme 1: EcoRI<br/>
Enzyme 2: SpeI<br/>
Enzyme 2: SpeI<br/>
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ligated vector was then transformed in cells and plated out on chloramphenicol plates.
ligated vector was then transformed in cells and plated out on chloramphenicol plates.
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<span style="color:blue;">Comments: The terminator between ho1 and pycA was missing. The digestion was repeated on june, 29.</span>
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==<span style="color:grey;">Precipitator</span>==
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===PCR of GFP fused to plastic-binding domain===
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'''Investigators: Sophie, Rüdiger'''
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S 14: GFP
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Primer:
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*GFPxb1Iup  (P1)<br/>
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*GFPpbdSpedw (P3)
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<span style="color:blue;">Comments: PCR did not work, because of too high concentration of primers. PCR was repeated on june, 30</span>
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{{:Team:Freiburg/Templates/footer}}

Latest revision as of 01:23, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

Digestion of minipreps for the green light receptor

Investigators: Sophie

Digestion of minipreps:

  • S 17 (Ccar 1:1)
  • S 18 (Ccar 1:3)
  • S 19 (Ccas 1:1)
  • S 20 (Ccas 1:3)
  • S 21 (artificial terminator)

Enzyme 1: XbaI Enzyme 2: PstI

two gels were loaded.

Comments: S17, S18 and S20 were ok. S21 was not digested.

red light receptor

3A assembly:Digestion, Ligation and Transformation for the receptor

Investigators: Julia

Digestion of ho1, pcyA (chromophor) and terminator (pSB1AK3).

Ho1 and pcyA:
Enzyme 1: EcoRI
Enzyme 2: SpeI

Terminator part (pSB1AK3):
Enzyme 1: EcoRI
Enzyme 2: XbaI

ligated vector was then transformed in cells and plated out on chloramphenicol plates.

Comments: The terminator between ho1 and pycA was missing. The digestion was repeated on june, 29.

Precipitator

PCR of GFP fused to plastic-binding domain

Investigators: Sophie, Rüdiger


S 14: GFP

Primer:

  • GFPxb1Iup (P1)
  • GFPpbdSpedw (P3)

Comments: PCR did not work, because of too high concentration of primers. PCR was repeated on june, 30

                       

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