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Team:Freiburg/Notebook/15 June
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| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/14_June">Previous entry</a> | ||
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| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook"> 15 June </a> | ||
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| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/16_June">Next entry</a> | ||
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==<span style="color:green;">green light receptor</span>== | ==<span style="color:green;">green light receptor</span>== | ||
===PCR for Ccas and CcaR=== | ===PCR for Ccas and CcaR=== | ||
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0,5 µl Phusion<br/> | 0,5 µl Phusion<br/> | ||
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| + | ===Gel with PCR products: Ccas, Ccar=== | ||
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| + | the first gel was stained with G-Stain, but adding G-Stain to the GeneRuler destroyed it. | ||
| + | Second Gel was post-stained with ethidium-bromid, the bands for CcaS &CcaR were at some place, nothing really clear. | ||
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| + | <span style= "color:blue;"> Comments: Has to be repeated. Either repeat the PCR or run a new gel with higher agarose concentration.</span> | ||
==Miniprep== | ==Miniprep== | ||
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| + | ==3A-assembly:Digestion== | ||
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| + | '''Investigators: Theo, Jakob''' | ||
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| + | Restriction digest of plasmid constructs (3A Assemblies PR1, PR2, PR3, PR4, PR5, PR7, PR8, Pr9)<br/> | ||
| + | Protocol: Mix => for one reaction, total volume 10µl<br/> | ||
| + | 3µl DNA<br/> | ||
| + | 1µl NEBuffer4<br/> | ||
| + | 1µl BSA<br/> | ||
| + | 0,5µl Enzym<br/> | ||
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==<span style="color:orange;">Lysis cassette</span>== | ==<span style="color:orange;">Lysis cassette</span>== | ||
===Transformation=== | ===Transformation=== | ||
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Latest revision as of 01:20, 22 September 2011
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This is the wiki page of the Freiburger student team competing for iGEM 2011. Thank you for your interest!
