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Team:Freiburg/Notebook/15 June
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| - | == | + | {{:Team:Freiburg/Templates/header}} |
| + | <html> | ||
| + | <div id="notebook-page-header"> | ||
| + | <div id="notebook-back" width="100px" > | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/14_June">Previous entry</a> | ||
| + | </div> | ||
| + | <div id="notebook-title"> | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook"> 15 June </a> | ||
| + | </div> | ||
| + | <div id="notebook-next"> | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/16_June">Next entry</a> | ||
| + | </div> | ||
| + | </div> | ||
| + | </html> | ||
| - | ==PCR for Ccas and CcaR== | + | ==<span style="color:green;">green light receptor</span>== |
| + | ===PCR for Ccas and CcaR=== | ||
'''Investigators: Julia''' | '''Investigators: Julia''' | ||
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0,5 µl Phusion<br/> | 0,5 µl Phusion<br/> | ||
<br/> | <br/> | ||
| - | < | + | |
| + | ===Gel with PCR products: Ccas, Ccar=== | ||
| + | |||
| + | the first gel was stained with G-Stain, but adding G-Stain to the GeneRuler destroyed it. | ||
| + | Second Gel was post-stained with ethidium-bromid, the bands for CcaS &CcaR were at some place, nothing really clear. | ||
| + | |||
| + | <span style= "color:blue;"> Comments: Has to be repeated. Either repeat the PCR or run a new gel with higher agarose concentration.</span> | ||
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==Miniprep== | ==Miniprep== | ||
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Miniprep PR4a, PR4b, PR8a, PR8b, PR9a, PR9b<br/> | Miniprep PR4a, PR4b, PR8a, PR8b, PR9a, PR9b<br/> | ||
measurement of nanodrop was ok<br/> | measurement of nanodrop was ok<br/> | ||
| + | <br/> | ||
| + | <br/> | ||
| + | ==3A-assembly:Digestion== | ||
| + | |||
| + | '''Investigators: Theo, Jakob''' | ||
| + | |||
| + | Restriction digest of plasmid constructs (3A Assemblies PR1, PR2, PR3, PR4, PR5, PR7, PR8, Pr9)<br/> | ||
| + | Protocol: Mix => for one reaction, total volume 10µl<br/> | ||
| + | 3µl DNA<br/> | ||
| + | 1µl NEBuffer4<br/> | ||
| + | 1µl BSA<br/> | ||
| + | 0,5µl Enzym<br/> | ||
| + | <br/> | ||
| + | |||
| + | ==<span style="color:orange;">Lysis cassette</span>== | ||
| + | ===Transformation=== | ||
| + | |||
| + | '''Investigators: Sandra''' | ||
| + | |||
| + | Transformation of BBa_K124014 bacteriophage Holin Gene pS105<br/> | ||
| + | plated out at 3:36pm in plates with ampicillin | ||
| + | <br/> | ||
| + | <br/> | ||
| + | |||
| + | '''Investigators: Julia''' | ||
| + | |||
| + | Transformation of BBa_E0040 green fluorescent protein. Ampicillin resistance. | ||
| + | <br/> | ||
| + | <br/> | ||
| + | {{:Team:Freiburg/Templates/footer}} | ||
Latest revision as of 01:20, 22 September 2011
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This is the wiki page of the Freiburger student team competing for iGEM 2011. Thank you for your interest!
