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Team:Freiburg/Notebook/11 June
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| - | == | + | {{:Team:Freiburg/Templates/header}} |
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| + | <div id="notebook-page-header"> | ||
| + | <div id="notebook-back" width="100px" > | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/10_June">Previous entry</a> | ||
| + | </div> | ||
| + | <div id="notebook-title"> | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook"> 11 June </a> | ||
| + | </div> | ||
| + | <div id="notebook-next"> | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/12_June">Next entry</a> | ||
| + | </div> | ||
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==Ligation of promotor, RBS in vector== | ==Ligation of promotor, RBS in vector== | ||
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<span style="color:blue;">Comments:For ligation digested samples were used from yesterday.</span> | <span style="color:blue;">Comments:For ligation digested samples were used from yesterday.</span> | ||
| - | Every promotor was ligated with every RBS. | + | Every promotor was ligated with every RBS using the Ginko Bioworks 3A-assembly protocol. |
PR1: S1 with S4 (660bp)<br/> | PR1: S1 with S4 (660bp)<br/> | ||
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PR8: S3 with S5<br/> | PR8: S3 with S5<br/> | ||
PR9: S3 with S6<br/> | PR9: S3 with S6<br/> | ||
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| + | Name for each construct: | ||
{| cellpadding="10" cellspacing="0" border="1" | {| cellpadding="10" cellspacing="0" border="1" | ||
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|S7 | |S7 | ||
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Latest revision as of 01:19, 22 September 2011
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This is the wiki page of the Freiburger student team competing for iGEM 2011. Thank you for your interest!
