Team:Freiburg/Notebook/11 June

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<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 11 June </a>
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==Ligation of promotor, RBS in vector==
==Ligation of promotor, RBS in vector==
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<span style="color:blue;">Comments:For ligation digested samples were used from yesterday.</span>
<span style="color:blue;">Comments:For ligation digested samples were used from yesterday.</span>
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Every promotor was ligated with every RBS.
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Every promotor was ligated with every RBS using the Ginko Bioworks 3A-assembly protocol.
PR1: S1 with S4 (660bp)<br/>
PR1: S1 with S4 (660bp)<br/>
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PR8: S3 with S5<br/>
PR8: S3 with S5<br/>
PR9: S3 with S6<br/>
PR9: S3 with S6<br/>
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Name for each construct:
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{| cellpadding="10" cellspacing="0" border="1"
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|
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|sample
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|name
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|-
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|Promotor
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|BBa_J23104
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|S1
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|-
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|Promotor
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|BBa_J23110
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|S2
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|-
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|Promotor
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|BBa_J23116
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|S3
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|-
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|RBS
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|BBa_B0034
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|S4
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|-
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|RBS
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|BBa_B0032
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|S5
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|-
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|RBS
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|BBa_B0031
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|S6
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|-
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|Vector
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|pSB1C3
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|S7
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|}
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Latest revision as of 01:19, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Ligation of promotor, RBS in vector

Investigators: Sophie, Theo, Sandra


Comments:For ligation digested samples were used from yesterday.

Every promotor was ligated with every RBS using the Ginko Bioworks 3A-assembly protocol.

PR1: S1 with S4 (660bp)
PR2: S1 with S5 (1000bp)
PR3: S1 with S6 (426bp)
PR4: S2 with S4 (233bp)
PR5: S2 with S5 (940bp)
PR6: S2 with S6 (981bp)
PR7: S3 with S4
PR8: S3 with S5
PR9: S3 with S6

Name for each construct:

sample name
Promotor BBa_J23104 S1
Promotor BBa_J23110 S2
Promotor BBa_J23116 S3
RBS BBa_B0034 S4
RBS BBa_B0032 S5
RBS BBa_B0031 S6
Vector pSB1C3 S7

                       

Retrieved from "http://2011.igem.org/Team:Freiburg/Notebook/11_June"