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Team:Freiburg/Notebook/11 June
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| - | == | + | {{:Team:Freiburg/Templates/header}} |
| + | <html> | ||
| + | <div id="notebook-page-header"> | ||
| + | <div id="notebook-back" width="100px" > | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/10_June">Previous entry</a> | ||
| + | </div> | ||
| + | <div id="notebook-title"> | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook"> 11 June </a> | ||
| + | </div> | ||
| + | <div id="notebook-next"> | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/12_June">Next entry</a> | ||
| + | </div> | ||
| + | </div> | ||
| + | </html> | ||
==Ligation of promotor, RBS in vector== | ==Ligation of promotor, RBS in vector== | ||
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<span style="color:blue;">Comments:For ligation digested samples were used from yesterday.</span> | <span style="color:blue;">Comments:For ligation digested samples were used from yesterday.</span> | ||
| - | Every promotor was ligated with every RBS. | + | Every promotor was ligated with every RBS using the Ginko Bioworks 3A-assembly protocol. |
| - | PR1: S1 with S4 (660bp) | + | PR1: S1 with S4 (660bp)<br/> |
| - | PR2: S1 with S5 (1000bp) | + | PR2: S1 with S5 (1000bp)<br/> |
| - | PR3: S1 with S6 (426bp) | + | PR3: S1 with S6 (426bp)<br/> |
| - | PR4: S2 with S4 (233bp) | + | PR4: S2 with S4 (233bp)<br/> |
| - | PR5: S2 with S5 (940bp) | + | PR5: S2 with S5 (940bp)<br/> |
| - | PR6: S2 with S6 (981bp) | + | PR6: S2 with S6 (981bp)<br/> |
| - | PR7: S3 with S4 | + | PR7: S3 with S4<br/> |
| - | PR8: S3 with S5 | + | PR8: S3 with S5<br/> |
| - | PR9: S3 with S6 | + | PR9: S3 with S6<br/> |
| + | |||
| + | Name for each construct: | ||
| + | |||
| + | {| cellpadding="10" cellspacing="0" border="1" | ||
| + | | | ||
| + | |sample | ||
| + | |name | ||
| + | |- | ||
| + | |Promotor | ||
| + | |BBa_J23104 | ||
| + | |S1 | ||
| + | |- | ||
| + | |Promotor | ||
| + | |BBa_J23110 | ||
| + | |S2 | ||
| + | |- | ||
| + | |Promotor | ||
| + | |BBa_J23116 | ||
| + | |S3 | ||
| + | |- | ||
| + | |RBS | ||
| + | |BBa_B0034 | ||
| + | |S4 | ||
| + | |- | ||
| + | |RBS | ||
| + | |BBa_B0032 | ||
| + | |S5 | ||
| + | |- | ||
| + | |RBS | ||
| + | |BBa_B0031 | ||
| + | |S6 | ||
| + | |- | ||
| + | |Vector | ||
| + | |pSB1C3 | ||
| + | |S7 | ||
| + | |} | ||
| + | {{:Team:Freiburg/Templates/footer}} | ||
Latest revision as of 01:19, 22 September 2011
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This is the wiki page of the Freiburger student team competing for iGEM 2011. Thank you for your interest!
