Team:Freiburg/Notebook/16 August

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<a href="https://2011.igem.org/Team:Freiburg/Notebook/15_August">Previous entry</a>
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<div id="notebook-title">
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<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 16 August </a>
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/17_August">Next entry</a>
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==<span style="color:green;">green light receptor</span>==
==<span style="color:green;">green light receptor</span>==
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'''Investigators:Julia'''
'''Investigators:Julia'''
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'''Digestion'''
'''Digestion'''
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*PR1 (pSB1C3)
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*PR1 in pSB1C3
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*PR2 (pSB1C3)
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*PR2 in pSB1C3
*pcyA (pSB1T3)
*pcyA (pSB1T3)
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#pcyATd (pSB1T3)
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#pcyA+terminator(d)in pSB1T3
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#pcyATb (pSB1T3)
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*L23 (pSB1T3)
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*ho1+Terminator (pSB1T3)
*vector (pSB1K3)
*vector (pSB1K3)
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===PCR===
===PCR===
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==<span style="color:orange;">Lysis cassette</span>==
==<span style="color:orange;">Lysis cassette</span>==
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===NAME OF YOUR EXPERIMENT===
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===2A Assembly like August 15===
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'''Investigators:NAME'''
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'''Investigators:Theo'''
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This time I used all the amount of S15 plasmid DNA we had, did an overnight restriction and run the gel.<br>
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[[File:Freiburg11 1608gelextraction.png]]
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<br>
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extraction as per SOP and ligation with S4 (cut with SpeI, PstI), Transformation in competent cells.
==<span style="color:grey;">Precipitator</span>==
==<span style="color:grey;">Precipitator</span>==

Latest revision as of 01:09, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!