Team:Freiburg/Notebook/15 August

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(NAME OF YOUR EXPERIMENT: Ligation)
 
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/14_August">Previous entry</a>
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<div id="notebook-title">
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<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 15 August </a>
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<div id="notebook-next">
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/16_August">Next entry</a>
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==<span style="color:green;">green light receptor</span>==
 
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===NAME OF YOUR EXPERIMENT===
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==<span style="color:green;">green light receptor</span>==
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'''Investigators:NAME'''
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===Order primers===
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'''Investigators: Jakob'''
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*Order new primers for the quickchange of CcaS and CcaR
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*I got the sequence from the iGEM-Team Uppsala THX again!
==<span style="color:blue;">blue light receptor</span>==
==<span style="color:blue;">blue light receptor</span>==
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==<span style="color:orange;">Lysis cassette</span>==
==<span style="color:orange;">Lysis cassette</span>==
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===NAME OF YOUR EXPERIMENT===
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===2A Assembly of the Lysis Cassette (S4+S15)===
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'''Investigators:NAME'''
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'''Investigators:Theo'''
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Since 3A assemly doesn't want to help us, we are going to try a 2A Assembly of S4 with S15.<br>
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S4 is cut with SpeI and PstI.<br>
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S15 is cut with XbaI and PstI, and is to be run on a gel, cut, isolated, ligated with S4 and finally transformed in competent cells.<br>
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After the gel with S15 was run, I noticed there was no band to be seen and our instructor told me it was because I had not used a lot of DNA (500ng).
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<br>
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I am going to repeat this tomorrow.
==<span style="color:grey;">Precipitator</span>==
==<span style="color:grey;">Precipitator</span>==
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===NAME OF YOUR EXPERIMENT: Ligation===
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===Ligation===
'''Investigators: Sophie'''<br/>
'''Investigators: Sophie'''<br/>
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Project name: inducible promoter for pbd<br/>
Project name: inducible promoter for pbd<br/>
Vector: psb1T3<br/>
Vector: psb1T3<br/>
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Inserts: IPTG-Promoter,Pbd-GFP
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Inserts: IPTG-Promoter,Pbd-GFP<br/>
samples stored in Ruediger's box<br/>
samples stored in Ruediger's box<br/>
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===NAME OF YOUR EXPERIMENT: Transformation===
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===Transformation===
'''Investigators: Sophie'''<br/>
'''Investigators: Sophie'''<br/>
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Project name: inducible promoter for pbd<br/>
Project name: inducible promoter for pbd<br/>
samples stored in incubator
samples stored in incubator
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===PCR===
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'''Investigators: Rüdiger'''
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PCR of precipitator.
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Primer:
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*p44
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*p45
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*p46
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*p48
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*51
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*52
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*53
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Primer:
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*p54
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*p47
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{| cellpadding="10" cellspacing="0" border="1"
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|name
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|primer
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|primer
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|-
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|44
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|p44
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|p54
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|-
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|45
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|p45
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|p54
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|-
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|46
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|p46
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|p54
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|-
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|48
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|p48
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|p47
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|-
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|51
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|p51
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|p54
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|-
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|52
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|p52
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|p54
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|-
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|53
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|p53
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|p54
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|}
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[[File:Freiburg_2011_Testdigest1.jpg|500px]]
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[[File:Freiburg_2011_tesdigest2.jpg|500px]]
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[[File:Freiburg_2011_testdigest3.jpg|500px]]
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{{:Team:Freiburg/Templates/footer}}
{{:Team:Freiburg/Templates/footer}}

Latest revision as of 01:08, 22 September 2011


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of the Freiburger student
team competing for iGEM 2011.
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