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Team:Freiburg/Notebook/2 August
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==<span style="color:green;">green light receptor</span>== | ==<span style="color:green;">green light receptor</span>== | ||
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===Miniprep=== | ===Miniprep=== | ||
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'''Documentation:''' | '''Documentation:''' | ||
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| - | + | * DNA concentration of CcaR a-d | |
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| - | + | Results, mistakes and DNA concentration measured with the Nanodrop. | |
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{| style="border-spacing:0;" | {| style="border-spacing:0;" | ||
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Sample ID | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Sample ID | ||
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| - | + | * Labeled and stored | |
| - | + | In Julias box, CcaR (qc.) a-d | |
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===Testdigest=== | ===Testdigest=== | ||
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Add 1 μl Loading dye buffer and load the gel. | Add 1 μl Loading dye buffer and load the gel. | ||
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===DNA Gel=== | ===DNA Gel=== | ||
| - | ''' | + | '''Investigator: Jakob''' |
[[File:Freiburg 11 8 2 2011 5 41 23 PM.jpg|400px]] | [[File:Freiburg 11 8 2 2011 5 41 23 PM.jpg|400px]] | ||
we dont see the expected edges.<br\> | we dont see the expected edges.<br\> | ||
| - | Repeat testdigest tomorrow. | + | *To-do: Repeat testdigest and if necessery quickchange tomorrow. |
==<span style="color:blue;">blue light receptor</span>== | ==<span style="color:blue;">blue light receptor</span>== | ||
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'''Investigators:Julia''' | '''Investigators:Julia''' | ||
| - | ==<span style="color: | + | ==<span style="color:grey;">Precipitator</span>== |
| - | === | + | ===Oligo order=== |
| - | '''Investigators: | + | '''Investigators: Rüdiger''' |
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{{:Team:Freiburg/Templates/footer}} | {{:Team:Freiburg/Templates/footer}} | ||
Latest revision as of 01:04, 22 September 2011
Contact 
This is the wiki page of the Freiburger student team competing for iGEM 2011. Thank you for your interest!
Contents |
green light receptor
transformation with quickchanged CcaS
Investigators:Julia
Miniprep
Investigator: Jakob
| Name:
Jakob | Date:
02.08.2011 |
| Continue from Experiment: 27.07.2011
Quickchange CcaR and trafo(28.07.2011) | |
| Project Name:
Green light receptor | |
Documentation:
- DNA concentration of CcaR a-d
Results, mistakes and DNA concentration measured with the Nanodrop.
| Sample ID | DNA conc. | Units | 260/280 | 260/230 |
| CcaR qc. a | 121,0 | ng/µl | 1,84 | 1,80 |
| CcaR qc. b | 121,1 | ng/µl | 1,87 | 2,05 |
| CcaR qc. c | 117,5 | ng/µl | 1,86 | 2,06 |
| CcaR qc. d | 121,4 | ng/µl | 1,87 | 2,08 |
- Labeled and stored
In Julias box, CcaR (qc.) a-d
Testdigest
Investigators: Julia
| Name:
Julia | Date:
02.08.2011 |
| Continue from Experiment 02.08.2011
Miniprep | |
| Project Name: green light receptor, testdigest of CcaR with BamHI | |
For one reaction you need: For Mastermix: Number of samples+2extra
| 4μl | H2O | 20 | |
| 1μl | Buffer, NEB4 | 5 | |
| 1μl | BSA (10x) | 5 | |
| 0,5 μl | Enzym 1 | 1 | BamHI |
| 0,5 μl | Enzym 2 | - | |
| 3 μl | DNA | 3 |
10 μl total volume
Give 3 μl of DNA in an eppi and add 7μl of the mastermix.
Incubate for about 1h at 37°C.
Add 1 μl Loading dye buffer and load the gel.
DNA Gel
Investigator: Jakob
we dont see the expected edges.
- To-do: Repeat testdigest and if necessery quickchange tomorrow.
blue light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
red light receptor
transformation with Ligationreaction(pcyA+terminator) from 1st of august and the pJT122
Investigators:Julia
Precipitator
Oligo order
Investigators: Rüdiger
