Team:Freiburg/Notebook/2 August

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{{:Team:Freiburg/Templates/header}}
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/1_August">Previous entry</a>
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<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 2 August </a>
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/3_August">Next entry</a>
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==<span style="color:green;">green light receptor</span>==
==<span style="color:green;">green light receptor</span>==
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'''Investigators:Julia'''
'''Investigators:Julia'''
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<br/>
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===Miniprep===
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'''Investigator: Jakob'''
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<br/>
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{| style="border-spacing:0;"
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Name:
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Jakob
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Date:
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02.08.2011
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|-
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| colspan="2"  style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Continue from Experiment: 27.07.2011
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Quickchange CcaR and trafo(28.07.2011)
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|-
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| colspan="2"  style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Project Name:
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Green light receptor
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|}
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'''Documentation:'''
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* DNA concentration of CcaR a-d
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Results, mistakes and DNA concentration measured with the Nanodrop.
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{| style="border-spacing:0;"
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Sample ID
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| DNA conc.
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Units
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 260/280
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 260/230
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|-
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| CcaR qc. a
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 121,0
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| ng/µl
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 1,84
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 1,80
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|-
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| CcaR qc. b
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 121,1
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| ng/µl
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 1,87
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 2,05
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|-
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| CcaR qc. c
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 117,5
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| ng/µl
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 1,86
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 2,06
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|-
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| CcaR qc. d
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 121,4
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| ng/µl
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 1,87
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 2,08
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|}
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* Labeled and stored
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In Julias box, CcaR (qc.) a-d
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<br/>
<br/>
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Add 1 μl Loading dye buffer and load the gel.
Add 1 μl Loading dye buffer and load the gel.
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<br/>
===DNA Gel===
===DNA Gel===
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'''Investirgators: Jakob'''
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'''Investigator: Jakob'''
[[File:Freiburg 11 8 2 2011 5 41 23 PM.jpg|400px]]
[[File:Freiburg 11 8 2 2011 5 41 23 PM.jpg|400px]]
we dont see the expected edges.<br\>
we dont see the expected edges.<br\>
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Reat testdigest tomorrow.
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*To-do: Repeat testdigest and if necessery quickchange tomorrow.
==<span style="color:blue;">blue light receptor</span>==
==<span style="color:blue;">blue light receptor</span>==
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'''Investigators:Julia'''
'''Investigators:Julia'''
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==<span style="color:orange;">Lysis cassette</span>==
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==<span style="color:grey;">Precipitator</span>==
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===NAME OF YOUR EXPERIMENT===
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===Oligo order===
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'''Investigators:NAME'''
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'''Investigators: Rüdiger'''
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==<span style="color:grey;">Precipitator</span>==
 
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===NAME OF YOUR EXPERIMENT===
 
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'''Investigators: NAME'''
 
{{:Team:Freiburg/Templates/footer}}
{{:Team:Freiburg/Templates/footer}}

Latest revision as of 01:04, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!


Contents

green light receptor

transformation with quickchanged CcaS

Investigators:Julia


Miniprep

Investigator: Jakob


Name:

Jakob

Date:

02.08.2011

Continue from Experiment: 27.07.2011

Quickchange CcaR and trafo(28.07.2011)

Project Name:

Green light receptor

Documentation:


  • DNA concentration of CcaR a-d

Results, mistakes and DNA concentration measured with the Nanodrop.


Sample ID DNA conc. Units 260/280 260/230
CcaR qc. a 121,0 ng/µl 1,84 1,80
CcaR qc. b 121,1 ng/µl 1,87 2,05
CcaR qc. c 117,5 ng/µl 1,86 2,06
CcaR qc. d 121,4 ng/µl 1,87 2,08


  • Labeled and stored

In Julias box, CcaR (qc.) a-d


Testdigest

Investigators: Julia


Name:

Julia

Date:

02.08.2011

Continue from Experiment 02.08.2011

Miniprep

Project Name: green light receptor, testdigest of CcaR with BamHI

For one reaction you need: For Mastermix: Number of samples+2extra


4μl H2O 20
1μl Buffer, NEB4 5
1μl BSA (10x) 5
0,5 μl Enzym 1 1 BamHI
0,5 μl Enzym 2 -
3 μl DNA 3

10 μl total volume


Give 3 μl of DNA in an eppi and add 7μl of the mastermix.

Incubate for about 1h at 37°C.


Add 1 μl Loading dye buffer and load the gel.


DNA Gel

Investigator: Jakob

Freiburg 11 8 2 2011 5 41 23 PM.jpg

we dont see the expected edges.

  • To-do: Repeat testdigest and if necessery quickchange tomorrow.

blue light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



red light receptor

transformation with Ligationreaction(pcyA+terminator) from 1st of august and the pJT122

Investigators:Julia

Precipitator

Oligo order

Investigators: Rüdiger


                       

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